Hitachi U-2810, Инструкция по эксплуатации

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2.5 2.5 Proper Proper Use Use of of Spectrophotome Spectrophotometer ter
2 - 8 2 - 8
2.5 2.5 Proper Proper Use Use of of Spectrophotometer Spectrophotometer
When selecting a solvent for When selecting a solvent for sample preparation, keep the sample preparation, keep the
following requirements in mind. following requirements in mind.
• •
Small Small absorption in absorption in measurement wavelength measurement wavelength range range
• •
Non-interactive Non-interactive with with solute solute
• •
Low Low volatility volatility
Table 2-1 lists the Table 2-1 lists the applicable wavelen applicable wavelength ranges of common gth ranges of common
organic solvents. organic solvents.
Table Table 2-1 2-1 Applicable Applicable Wavelength Wavelength Ranges Ranges
(indicated by solid lines) (indicated by solid lines)
Wavelength Wavelength
Solvent Solvent
Cyclohexane Cyclohexane
Ethyl alcohol Ethyl alcohol
Methyl alcohol Methyl alcohol
Ethyl ether Ethyl ether
Dioxane Dioxane
Hexane Hexane
Chloroform Chloroform
Isopropyl alcohol Isopropyl alcohol
Acetic acid Acetic acid
Ethyl acetate Ethyl acetate
C Ca arrb bo on t n te etr tra ac ch hllo orriid de e
Benzene Benzene
Methyl ethyl ketone Methyl ethyl ketone
Acetone Acetone
Carbon disulfide Carbon disulfide
Note that equations (1) and (2) in 2.3 are not Note that equations (1) and (2) in 2.3 are not applicable to the applicable to the
following special samples. following special samples.
• •
Fluorescing Fluorescing sample sample
• •
Appreciably Appreciably turbid turbid sample sample
In measurement of a solid sample such as glass plate, the In measurement of a solid sample such as glass plate, the
energy of beam radiation undergoes a loss due
energy of beam radiation undergoes a loss due to reflection on to reflection on
the the surface surface of s of solid olid substance. substance. In th In this ca is case, th se, the fol e following lowing
equation is set up. equation is set up.
II
tt
/I /I
0 0
= 10 = 10
--
ε⋅ ε⋅
c c
⋅⋅
ll
- r....................................................(3) - r....................................................(3)
where, r varies according to where, r varies according to the substance. the substance.
2.5.1 2.5.1 Selection Selection of of
Solvent Solvent
2.5.2 2.5.2 Special Special Samples Samples
400 nm 400 nm
2 20 00 0 n nm m 3 30 00 0 n nm m
250 nm 250 nm
200 nm 200 nm
220 nm 220 nm
220 nm 220 nm
220 nm 220 nm
220 nm 220 nm
220 nm 220 nm
250 nm 250 nm
250 nm 250 nm
270 nm 270 nm
275 nm 275 nm
280 nm 280 nm
335 nm 335 nm
340 nm 340 nm
380 nm 380 nm