3.1
3.1
3 - 1
3 - 1
3.
3. INSTRUMENT
INSTRUMENT CALIBRATION
CALIBRATION
3.1 Introduction
3.1 Introduction
This section describes how to calibrate the
This section describes how to calibrate the instrument.
instrument.
0%T measurement is intended for calibration in the
0%T measurement is intended for calibration in the ordinate
ordinate
direction.
direction.
Wavelength calibratio
Wavelength calibration is
n is intended for calibration in the abscissa
intended for calibration in the abscissa
direction.
direction.
Zero %T measurement must be carried
Zero %T measurement must be carried out in the
out in the following cases.
following cases.
•
•
For accurate
For accurate measuremen
measurement of a
t of a sample hi
sample high in
gh in absorbance
absorbance
(low in
(low in transmittance)
transmittance)
•
•
When an
When an error messa
error message “Calib
ge “Calibrate!” appea
rate!” appears as sh
rs as shown in
own in
Fig. 3-1
Fig. 3-1
Fig. 3-1
Fig. 3-1
NOTICE
NOTICE
:
: Before
Before carrying
carrying out
out instrument
instrument calibration,
calibration, wait
wait
until the spectrophotometer is fully stabilized (at
until the spectrophotometer is fully stabilized (at
least 2 hours after turning on power).
least 2 hours after turning on power).
Conduct instrument calibration in the order of
Conduct instrument calibration in the order of
zero %T
zero %T measuremen
measurement,
t, wavelength calibration
wavelength calibration
and baseline measurement (system).
and baseline measurement (system).
Error message
Error message
