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1.3 High-Pressure Gradient System
In the high-pressure gradient system, two or more solvents are
fed by respective pumps and mixed in the high-pressure section
for liquid delivery.
(1) From the degasser, each of plural eluents is applied to the
pump.
(2) The plural eluents are fed at a flow rate corresponding to a
pre-specified mixing ratio.
(3) The plural eluents thus fed are mixed in the high-pressure
gradient section. Through the mixer, the mixture eluent is
delivered.
(4) A sample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column
oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
1.3 High-Pressure Gradient System
Eluent
Drain
Model L-2100/
2130 pump
Model L-2200
autosampler
Model L-2300
column oven
Model L-2400
UV detector
System power
supply unit
Model L-2100/
2130 pump
Washing
solution
Mixer
Degasser
Column
