Chapter 4: Software Interface
Exicor MicroImager User Manual
27
Offsets
The offset button is found just below the sample button. Clicking this button will take
map the current retardation and angle of the image and then store it in memory to be
subtracted later.
After you take an offset, you can choose to use the offset or not by checking the box
next to the offset button.
Each color/objective combination will have its own unique offset. For example, if you
create an offset for a 2x objective using the red LED, you will only be able to use that
offset when the software is set to the red LED and the 2x objective.
Display of data
Data is displayed in 7 Different formats. Intensity, Retardance, Angle, Retardance
and Angle, Retardance and Intensity, Angle and Intensity, and all three together.
When displaying single sets of data the color map in the upper right corner provides
the legend to change the color scheme of the image. Clicking on the color map
brings up the screen shown in Figure 11.
Figure 11 Colorbar Picker.
To change between preset color configurations, such as grayscale, right click on the
left colored part and choose the color scheme desired.
To create a custom color scheme, you can add colors by clicking on the slider bar.
To choose what color you want a particular marker to be, click on it and then move
the selector in the hue generator to pick the color you would like.
To choose the maximum and minimum values of the color map, it is possible to
either use an auto setting by clicking the Auto max/min or Full max/min buttons on
the right side of the screen or setting custom values. Auto max/min will use the
pixels with the lowest and highest values to determine the maximum and minimum.
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