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ElectroPrep System
Publication 9511-073 REV-1.0
ElectroPrep Protocols
Selective Electrofiltration/Concentration/Separation Based on
Different Charges of Biomolecules (Variation of Configuration #2)
Applications
• Separation and purification of biomolecules with unknown isoelectric potential
In Configuration #2 the sample is placed in a union between two membranes (b), both of which should
have a MWCO larger than the desired biomolecules. Membranes (a) and (c) should have MWCOs smaller
than the desired biomolecules.
Based on their charges, the desired biomolecules will move to either dialysis Chamber (1) or Chamber
(2), whereas the lowest molecular weight molecules will migrate through membranes (a) and (c) into
the tank. Biomolecules with unknown isoelectric points can also be separated and purified using this
method. Dialysis chambers of smaller volume can be used to concentrate samples.
Steps
1. Select the proper size Dialyzer Chamber and Union to use as much of the available chamber volume as
possible (minimize air gaps).
2. Assemble Dialyzer Unit by placing one membrane (a) on the platform of the Dialyzer Chamber and
hand-tightening an End Cap.
3. Add buffer to the Dialyzer chamber.
4. Place your next membrane (b) on the other side of the Dialyzer Chamber and assemble the Union.
5. Add your sample to the Union compartment and place your next membrane (b) and Dialyzer Chamber.
6. Add buffer to the second Dialyzer Chamber, place the last membrane (c), and place End Cap
to create the completed Dialysis Chamber unit.
7. Gently push the Dialysis Chamber unit through the ElectroPrep tank gasket to secure the unit in place.
8. Fill the Electroprep tank with buffer and ensure the Dialysis Chambers are covered, but the fluid does
not flow over the partition. Chambers and caps must be completely immersed in buffer so that no air
bubbles are present. Keeping all parts submersed in buffer prevents the introduction of air bubbles
into the Dialyzer Chambers.
9. Assemble the lid with cables and connect to your power supply. Use current and voltage settings as
required for the sample and buffer (5 to 10 mA suggested as a starting point).
ElectroPrep Dialyzer
Open End Cap
Membrane
Union
Key
Selective Electro-Filtration/
Concentration/Separation
Based on Different Charges
on Biomolecules
