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ElectroPrep System

Publication 9511-073 REV-1.0

Using the ElectroPrep Unit

•

Dialysis Membranes are added at one or both ends or between Dialysis Chamber

and/or Link Chambers and Unions. Membranes with molecular weight cut off

points (MWCOs), ranging from 500 to 300,000 daltons, may be used in combination

with different Dialysis and Link Chambers for selective elution, filtration, dialysis,

fractionation and concentration of complex samples. Dialysis Membranes are available

in three sizes: 7/16”, 11/16”, and 15/16” Membrane diameters are available. The

Ordering Information at the end of this manual indicates what diameter membrane is

used for various components and chamber sizes.

NOTE: ElectroPrep dialysis chambers, unions, link chambers and membranes are

purchased separately. Components required depend on configuration.

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Содержание QuikPrep ElectroPrep 74-1196

Страница 1: ...Publication 9511 073 REV 1 0 ElectroPrep System USER S MANUAL ElectroPrep System 74 1196 ...

Страница 2: ...ane Configuration 10 11 ElectroPrep Protocols 12 15 Electrodialysis through Simultaneous Exchange of Buffers 12 Selective Electrofiltration Concentration Separation Based on Different Charges of Biomolecules 13 Rapid and Selective Electrofiltration or Concentration 14 Electroelution and Electrofractionation of DNA Proteins or Other Biomolecules from Gel Pieces 15 Specifications 16 General Care Mai...

Страница 3: ...o not allow this limitation on an implied warranty so the above limitation may not apply to you Without limiting the generality of the foregoing Harvard Apparatus shall not be liable for any claims of any kind whatsoever as to the equipment delivered or for non delivery of equipment and whether or not based on negligence Warranty is void if the instrument is changed in any way from its original fa...

Страница 4: ...hen ordering please describe parts as completely as possible preferably using our part numbers If practical enclose a sample photo or drawing Safety Precautions Always isolate ElectroPrep unit from the power supply before removing the safety cover Isolate the power supply from the main first then disconnect the leads DO NOT exceed the maximum operating voltage or current DO NOT operate the unit in...

Страница 5: ...osed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the product do not operate the product Contact qualified service personnel to perform inspection Orient the Equipment Properly Do not orient the equipment so that is difficult to manage the connection and disconnection of devices Place Product in Proper ...

Страница 6: ...icable safety requirements for electrical equipment for measurement control and laboratory use The unit itself does not generate waste but may be used to treat samples that are hazardous Please use appropriate PPE and ensure disposal in accordance with local regulations and practices This product should not be used in the presence of a flammable atmosphere such as an anesthetic mixture with air ox...

Страница 7: ...tion The ElectroPrep System provides faster dialysis times due to movement of charged molecules in an electric field during dialysis thus combining electrophoresis with dialysis With a run time of 5 to 10 minutes ElectroPrep provides speed and convenience even at the very low currents 5 to 10 mA used with this system Applications Electroelution from gels and solutions e g gel extraction of vector ...

Страница 8: ... with a tank lid and connectors and a replacement gasket Power supply and Dialyzer Units must be purchased separately The ElectroPrep System uses at least one Dialyzer Unit to perform a sample electrodialysis A basic Dialyzer Unit is comprised of a dialyzer chamber dialysis membranes at one or both ends of the chamber and two end caps Example of basic Dialyzer Unit unassembled comprised of one dia...

Страница 9: ...yzed samples or for size fractionation of samples using membranes of different MWCOs As with Unions Link Chambers may also be connected to Chambers without membranes to make a larger volume chamber Each Link Chamber comes with one open end cap Primary and Secondary Link Chambers accept different size membranes at their junctions on either side facing the Dialysis Chamber or the Link Chamber cap Pr...

Страница 10: ...th different Dialysis and Link Chambers for selective elution filtration dialysis fractionation and concentration of complex samples Dialysis Membranes are available in three sizes 7 16 11 16 and 15 16 Membrane diameters are available The Ordering Information at the end of this manual indicates what diameter membrane is used for various components and chamber sizes NOTE ElectroPrep dialysis chambe...

Страница 11: ...ay be used at junctions of these chambers and caps Primary Link Chamber Can be connected to a Dialysis Chamber and joined with a Secondary Link Chamber or Open End Cap at the other end Membranes of different MCWOs may be used at junctions of these chambers and caps for size fractionation How to Select Your Chamber and Membrane Configuration 1 Decide upon your application e g electrodialysis electr...

Страница 12: ...membranes a smaller volume Link Chamber and two Open End Caps for electroconcentration or electrofiltration Configuration 3 With Dialysis Chambers six membranes of different MWCO a Union and multiple Link Chambers for electrofractionation Configuration 4 Note Configurations 1 to 4 are just a few examples of Electroprep Unit assembly Additional configurations for electrofractionation are possible u...

Страница 13: ...e biomolecule s of interest 3 Assemble Dialyzer Unit by placing one membrane on the platform of the Dialyzer Chamber and hand tightening one End Cap 4 Place the sample in the Dialysis Chamber between the two membranes 5 Place the second membrane and assemble the second End Cap to create the completed Dialysis Chamber unit 6 Install the Dialysis Chamber unit into the tank by gently pushing the unit...

Страница 14: ...2 Assemble Dialyzer Unit by placing one membrane a on the platform of the Dialyzer Chamber and hand tightening an End Cap 3 Add buffer to the Dialyzer chamber 4 Placeyournextmembrane b ontheothersideoftheDialyzerChamberandassembletheUnion 5 AddyoursampletotheUnioncompartmentandplaceyournextmembrane b andDialyzerChamber 6 Add buffer to the second Dialyzer Chamber place the last membrane c and place...

Страница 15: ...hamber 2 and hand tightening an End Cap 3 Add buffer to the Dialyzer Chamber 4 Place your next membrane b on the other side of the Dialyzer Chamber and assemble the Union to create a combined large compartment 5 Assemble second Dialyzer Chamber 1 to the Union and add your sample to the combined large compartment 6 Placeyournextmembrane c ontheothersideofthecombinedchamberandassembleEndCap 7 Gently...

Страница 16: ...and tighten 3 Add buffer to the elution sample collection Dialyzer Chamber 4 Place membrane b MWCO larger than your biomolecule into the elution sample collection Dialyzer Chamber Add Union onto other end 5 Assemble second Dialyzer Chamber 1 to the Union to create a combined large compartment and add gel slice s and buffer to the combined large compartment 6 Place your next Membrane c lower MWCO t...

Страница 17: ...Category II ENVIRONMENTAL CONDITIONS Location Indoor use only Operation Ambient Temperature 5 to 40 C Operation Maximum Relative Humidity 80 non condensing Operation Altitude 2000 m Shipping and Transportation Ambient Temperature 20 to 60 C Shipping and Transportation Maximum Relative Humidity 95 non condensing Pollution Degree 2 The apparatus is rated POLLUTION DEGREE 2 in accordance with IEC 606...

Страница 18: ...heet of dry tissue and then fill with DISTILLED WATER ONLY to the maximum fill line Any leakage will be seen on the tissue paper If any leakage is seen DO NOT ATTEMPT TO REPAIR OR USE THE APPARATUS but notify Harvard Apparatus immediately The replacement platinum electrodes are partially shrouded for protection However when cleaning the main tank DO NOT use cleaning brushes in the electrode area U...

Страница 19: ...amber Volume 7411 15002D Link Chambers Qty of 2 50 µl Chamber Volume 1 11 16 dia Primary link and 1 7 16 dia Secondary link 7411 502L 100 µl Chamber Volume 1 11 16 dia Primary link and 1 7 16 dia Secondary link 7411 1002L 250 µl Chamber Volume 2 11 16 dia Primary links 7411 2502L 500 µl Chamber Volume 2 11 16 dia Primary links 7411 5002L 1000 µl Chamber Volume 2 11 16 dia Primary links 7411 10002L...

Страница 20: ...WCO 7410 CA100K 300 kDa MWCO 7410 CA300K Polycarbonate 0 01 µm Pore Size 7410 PC01 0 05 µm Pore Size 7410 PC05 0 10 µm Pore Size 7410 PC10 0 60 µm Pore Size 7410 PC60 ElectroPrep Membranes for Primary Link Chambers 50 to 250 µl 11 16 Diameter Type and MWCO Regenerated Cellulose 1 kDa MWCO 7416 RC1K 2 kDa MWCO 7416 RC2K 3 5 kDa MWCO 7416 RC3 5K 10 kDa MWCO 7416 RC10K 25 kDa MWCO 7416 RC25K 50 kDa M...

Страница 21: ...C10 0 60 µm Pore Size 7416 PC60 ElectroPrep Membranes for Primary Link Chambers 500 to 1500 µl 11 16 Diameter Type and MWCO Regenerated Cellulose 1 kDa MWCO 7425 RC1K 2 kDa MWCO 7425 RC2K 3 5 kDa MWCO 7425 RC3 5K 10 kDa MWCO 7425 RC10K 25 kDa MWCO 7425 RC25K 50 kDa MWCO 7425 RC50K Cellulose Acetate 500 Da MWCO 7425 CA500 1 kDa MWCO 7425 CA1K 2 kDa MWCO 7425 CA2K 5 kDa MWCO 7425 CA5K 10 kDa MWCO 74...

Страница 22: ... Regenerated Cellulose 1 kDa MWCO 7424 RC1K 2 kDa MWCO 7424 RC2K 3 5 kDa MWCO 7424 RC3 5K 10 kDa MWCO 7424 RC10K 25 kDa MWCO 7424 RC25K 50 kDa MWCO 7424 RC50K Cellulose Acetate 500 Da MWCO 7424 CA500 1 kDa MWCO 7424 CA1K 2 kDa MWCO 7424 CA2K 5 kDa MWCO 7424 CA5K 10 kDa MWCO 7424 CA10K 25 kDa MWCO 7424 CA25K 50 kDa MWCO 7424 CA50K 100 kDa MWCO 7424 CA100K 300 kDa MWCO 7424 CA300K Polycarbonate 0 01...

Страница 23: ...22 ElectroPrep System Publication 9511 073 REV 1 0 Notes ...

Страница 24: ...23 ElectroPrep System Publication 9511 073 REV 1 0 Notes ...

Страница 25: ...e 508 893 8999 Toll Free 800 272 2775 Fax 508 429 5732 E mail support hbiosci com Web www harvardapparatus com Germany Hugo Sachs Elektronik Gruenstrasse 1 March Hugstetten D 79232 Germany Phone 49 0 7665 92 00 0 Fax 49 0 7665 92 00 90 E mail info hugo sachs de Web www hugo sachs de France Harvard Apparatus S A R L 6 Avenue des Andes Miniparc Bat 8 F 91952 Les Ulis Cedex Phone 33 1 64 46 00 85 Fax...

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