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4: Sample Experiments
Where:
S
λ
= the sample intensity at wavelength
λ
D
λ
= the dark intensity at wavelength
λ
R
λ
= the reference intensity at wavelength
λ
The following figure shows a typical reflection setup. A light source sends light via the input leg of a
reflection probe onto a sample. A reflection probe holder holds the probe in either a 90 or 45-degree angle
from the surface. The output leg of the reflection probe carries light from the sample to the spectrometer,
which is connected to the PC.
Typical Reflection Setup
Common reflection applications include measuring the properties of mirrors and coatings. Other
applications include measuring the visual properties of the color in paints, plastics, and food products.
►
Procedure
Perform the following steps to take reflection measurements using OOIBase32:
1. Place OOIBase32 is in Scope mode by clicking the
Scope Mode
icon on the toolbar, or by
selecting
Spectrum | Scope Mode
from the menu bar.
2. Ensure that the entire signal is on scale. The intensity of the reference signal should peak at about
50,000 counts.
3. Take a reference spectrum with the WS-1 Diffuse Reflectance Standard or the STAN-SSH High-
reflectivity Reference Standard. You must take a reference spectrum before measuring reflection.
Click the
Store Reference
spectrum icon on the toolbar or select
Spectrum | Store Reference
from the menu bar to store the reference. This command merely stores a reference spectrum in
memory. You must select
File | Save | Reference
from the menu bar to permanently save the
spectrum to disk.
4. Block the light path to the spectrometer. Then, take a dark spectrum by clicking the
Store Dark
Spectrum
icon on the toolbar or by selecting
Spectrum | Store Dark
from the menu bar. This
command merely stores a dark spectrum in memory. You must select
File | Save | Dark
from the
menu to permanently save the spectrum to disk.
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Содержание Ocean Optics QE65000
Страница 6: ...Table of Contents iv 220 00000 000 02 0605 ...
Страница 14: ...1 Introduction 6 220 00000 000 02 0605 ...
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