Gilson PLATEMASTER P20, Руководство пользователя

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Volume Setting
Do not adjust the volume without squeezing both parts of the pipetting handle
 .
The volume of liquid to be aspirated is set
using the volume adjustment wheel
 .
With the pipetting handle fully squeezed,
the volume is set by slowly turning the
volume adjustment wheel to reach the
required setting.
Never try to force the volume outside of
the volume range permitted by the device.
Tip Fitting
For optimum performance, use of PIPETMAN® DIAMOND Tips with PLATEMASTER is
strongly recommended. Plastic tips are for a single use and should not be cleaned for
reuse.
To fit PIPETMAN® DIAMOND Tips on PLATEMASTER:
• Raise the pipetting head vertically
using the pipetting handle. Move the
pipetting head

to the left side.
• Slide the right drawer out

and place
a new tip rack on the back position.
The four locations have been specially
designed for PIPETMAN DIAMOND
Tips TIPACK™.
Slots embedded in
the drawers ensure that tip racks
securely fit into all locations.
• Use the pipetting handle

to position
the pipetting head

to the right and
lower it far enough so that a contact is
made with the tips in the rack.
• Press down on the tip fitting handle

with moderate pressure. The tip ejector must be fully expanded to ensure that the
tips are fully attached.

.
• Move the pipetting head

up and the right drawer

back.
The device is now ready to pipette liquid.


5
3
1
4
2
Pipetting
Place a vessel containing the solution to be pipetted in the front location of the right
drawer. The solution should be under the pipetting head.
Liquids containing protein solutions and organic solvents can leave a film of liquid
on the inside wall of the tip; pre-rinse the tip to minimize any errors related to this
phenomenon. Pre-rinsing consists of aspirating the first volume of liquid and then
dispensing it back into the same vessel (or to waste). Subsequent volumes pipetted
will have levels of accuracy and precision within specifications. As recommended by
the ISO 8655 standard, pre-rinse to stabilize dead volume inside the pipetting head.
ASPIRATION
• Gently squeeze the pipetting handle

to the first stop (this corresponds
to the set volume of liquid).
• Use the pipetting handle

to lower
the pipetting head

to immerse the
tips in the liquid (see immersion depth
table below).
It is important that the
tips do not touch the bottoms of the
wells.
• Slowly retract the pipetting handle to
aspirate the liquid into the tips.
• Move the pipetting head fully up.
To simplify this operation, the height
adjuster

can be set by turning the upper
screw so that the pipetting head may only be
moved down to a position slightly above the
bottom.



MODEL
IMMERSION DEPTH
(MILLIMETERS)
WAIT TIME
(SECONDS)
P20 2-3 1
P220 2-4 1
Check the tip-ejector when fitting tips. If the tips have shifted this
implies that they have not been fitted correctly.
The maximum
immersion depth
of the tips can be
adjusted using a
height adjuster.
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PLATEMASTER®
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