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GE ImageQuant LAS 4000, Руководство пользователя

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4.7 Exposing fluorescence (Epi illumination)

This section describes how to expose fluorescent samples using Epi illumination.

Ensure that the correct Epi lights and filter are installed. See

Chapter 3 Exchanging accessory parts, on

page 17

.

Place a sample on the Epi tray. Put the tray into the ImageQuant LAS 4000 and close the door.

Note:

• Make sure that sample is suitably placed on the tray for the desired tray position.

• Ensure that a suitable method exists and that the ImageQuant LAS 4000 is properly

calibrated. See

Section 4.16 Creating a new method and performing flat frame

calibration, on page 78

.

5

4

3

6

2

1

ImageQuant LAS 4000 User Manual 28-9607-42 AB

47

4 Operating the ImageQuant LAS 4000

4.7 Exposing fluorescence (Epi illumination)

Содержание ImageQuant LAS 4000

Страница 1: ...GE Healthcare ImageQuant LAS 4000 User Manual ...

Страница 2: ......

Страница 3: ... the ImageQuant LAS 4000 Control Software 33 4 2 Preparation for exposure 34 4 3 Calibration 34 4 4 Placing the sample 37 4 5 Exposing chemiluminescent samples 42 4 6 Exposing fluorescent samples EtBr 47 4 7 Exposing fluorescence Epi illumination 51 4 8 Exposing dye stained samples and films White Epi light 55 4 9 Exposing dye stained samples and films Trans illumination 59 4 10 Exposing consecuti...

Страница 4: ...ImageQuant LAS 4000 Control Software under Windows XP 113 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista 118 7 Uninstalling and upgrading ImageQuant LAS 4000 Control Software 120 8 After sales service 120 8 1 Warranty 120 8 2 Repairs 121 A Appendix 121 A 1 Glossary 122 A 2 Quick reference functions 125 A 3 Detection Reagents and corresponding settings 127 A 4 Main specificati...

Страница 5: ...se of this manual The ImageQuant LAS 4000 User Manual provides detailed instructions on using the ImageQuant LAS 4000 and outlines the functions of the ImageQuant LAS 4000 Control Software Note Be sure to refer also to the manual Getting Started with ImageQuant LAS 4000 for regulatory information specifications and the troubleshooting guide 1 1 Important user information Read this before using the...

Страница 6: ... It is important not to proceed until all stated conditions are met and clearly understood Cautions CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood Notices NOTICE NOTICE indicates instructions that must be followed to avoid damage to the product or...

Страница 7: ...ul interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense This Class A digital apparatus complies with Canadian ICES 003 Cet appareil numérique de la classe A conforme à la norme NMB 003 du Canada Note This is a class A product In a domestic...

Страница 8: ...avelength 710nm NIR Epi light Class 1 Wavelength 470 740nm White Epi light CAUTION Do not look at the light directly through optical instruments CAUTION If the door is opened and the interlock is cancelled class 1 laser LED will be emitted CAUTION Use of controls or adjustments or performance of procedures other than those specified in the user documentation may result in hazardous radiation expos...

Страница 9: ... bold italic text A colon separates menu levels thus File Open refers to the Open command in the File menu Hardware items are identified in the text by bold text e g Power switch ImageQuant LAS 4000 User Manual 28 9607 42 AB 9 1 Introduction 1 1 Important user information ...

Страница 10: ... exterior 6 7 1 2 3 4 5 Description Name Part CCD cooling and image data output Camera head 1 Status lights Indicator panel 2 Lights when the power is on Power LED 3 Lights when an exposure is in progress Busy LED 4 Lights when an error is detected Error LED 5 Power ON I Power OFF O Power switch 6 Dark box Intelligent dark box IDX 7 10 ImageQuant LAS 4000 User Manual 28 9607 42 AB 2 The ImageQuant...

Страница 11: ...lue Epi light 460 nm RGB Module Epi light with red green and blue LEDs UV Epi light 365 nm White Epi light Supports the sample Sample tray 3 Moves the sample to the selected tray position Lifting table 4 F0 85 43 mm LAS High Sens lens or Lens 5 F1 8 24 mm wide view lens Connects lens to IDX Lens connector 6 ImageQuant LAS 4000 User Manual 28 9607 42 AB 11 2 The ImageQuant LAS 4000 2 2 Inside the I...

Страница 12: ...IDX Trans light source connec tor 8 312 nm UV transilluminator Trans light source 9 White light table Note Do not use connectors other than those supplied for the Epi light sources Note Do not connect cables other than those supplied to the lens Note Do not connect anything other than the transmitted light sources to the transmitted light source connector 2 3 Connections Camera head 2 1 Name No Ca...

Страница 13: ...ce connector inside the IDX Epi light source connector Figure 2 2 Inside the instrument A connector used for connecting the Epi light source Power is supplied to the Epi light source through this connector Note The Epi light sources for the right and left sides are different Attach the appropriate source on respective sides The connector will be connected when the Epi light source is inserted Make...

Страница 14: ...he USB cable supplied with the equipment Do not connect the instrument and the PC via a USB hub Do not connect USB equipment other than ImageQuant LAS 4000 to the PC Simi larly do not use other USB equipment connected to the PC while ImageQuant LAS 4000 is in use This may result in the loss of image data The PC must be certified according to UL60950 1 UL listed and IEC60950 1 2 4 Parts and accesso...

Страница 15: ...te FL Green NP tray White trans tray UV trans tray White light table Gel sheet UV transilluminator F mount adapter F1 8 24mm wide view lens ImageQuant LAS 4000 User Manual 28 9607 42 AB 15 2 The ImageQuant LAS 4000 2 4 Parts and accessories ...

Страница 16: ...lexa Fluor 750 detection filter IR785 Alexa Ultraviolet protection filter L41 UV Note The combined accessories and light source vary depending on the system that you purchase Make sure to retain the caps and filter cases Store the incident Epi light sources in a container box The UV trans tray will degrade after many UV illuminations It can be used up to approximately 1000 times if each exposure t...

Страница 17: ...change a filter on the filter turret Install a filter in the filter turrent and register it in the ImageQuant LAS 4000 Control Software as follows 3 2 5 6 4 1 Ensure that the camera head and PC are connected Turn on the instrument and the PC and start the ImageQuant LAS 4000 Control Software 2 Close the instrument door ImageQuant LAS 4000 User Manual 28 9607 42 AB 17 3 Exchanging accessory parts ...

Страница 18: ... the drop down list and click the OK button Note The filter changer returns to the original position when you click the Start Focusing or Method Tray position buttons 3 2 Installing or exchanging the lens Two lenses can be used with the ImageQuant LAS 4000 an F0 85 43mm LAS High Sens lens and an optional F1 8 24mm wide view lens The wide view lens can image samples up to 250 x 250 mm in size For a...

Страница 19: ...er is turned off and the filter changer is down proceed to step 4 2 Select Option Lens Exchange A message is displayed 3 Click OK 4 Turn off the instrument using the power switch ImageQuant LAS 4000 User Manual 28 9607 42 AB 19 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 1 Removing the LAS High Sens lens ...

Страница 20: ...y rotating the knurling ring Connector of the lens cable Knurling ring 7 Turn the lens slightly to the left and loosen it while pushing the lens release lever Lens release lever 20 ImageQuant LAS 4000 User Manual 28 9607 42 AB 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 1 Removing the LAS High Sens lens ...

Страница 21: ... hands so as to prevent the lens guide from being damaged 9 Take out the lens slowly along the lens guide toward you 10 Place the end cap on End cap ImageQuant LAS 4000 User Manual 28 9607 42 AB 21 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 1 Removing the LAS High Sens lens ...

Страница 22: ... high sensitivity lens weighs 4 5 kg Be careful when handling it 3 2 2 Installing the LAS High Sens lens 1 Close the instrument door If the power is turned off and the filter changer is down proceed to step 4 2 Select Option Lens Exchange A message is displayed Click OK 3 Turn off the instrument using the power switch 4 Remove the end cap from the lens 5 Insert the lens along the lens guide with t...

Страница 23: ...curely fixed Connector of the lens cable Knurling ring Note Be sure to connect the lens cable Otherwise the high sensitivity lens cannot be recognized This may cause malfunction of the equipment ImageQuant LAS 4000 User Manual 28 9607 42 AB 23 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 2 Installing the LAS High Sens lens ...

Страница 24: ... lens 1 Close the instrument door If the power is turned off and the filter changer is down proceed to step 4 2 Select Option Lens Exchange 24 ImageQuant LAS 4000 User Manual 28 9607 42 AB 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 2 Installing the LAS High Sens lens ...

Страница 25: ...t 6 Remove the lens hood from the lens 7 Place the lens cap on 8 Place the end cap on the lens and the cap on the F mount adapter 3 2 4 Installing the wide view lens 1 Close the instrument door If the power is turned off and the filter changer is down proceed to step 4 2 Select Option Lens Exchange ImageQuant LAS 4000 User Manual 28 9607 42 AB 25 3 Exchanging accessory parts 3 2 Installing or exch...

Страница 26: ...emove the end cap from the wide view lens 6 Align the White point mark of the F mount adapter with the lens mark and turn to lock them together Hold the lens so that the red point is towards you F mount adapter Wide view lens 26 ImageQuant LAS 4000 User Manual 28 9607 42 AB 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 4 Installing the wide view lens ...

Страница 27: ...le for exposure with the ImageQuant LAS 4000 This section describes how to change both types of light source The following table summarizes the properties of the various light sources Peak wavelength Name Type 312 nm UV transilluminator Transmitted White light table ImageQuant LAS 4000 User Manual 28 9607 42 AB 27 3 Exchanging accessory parts 3 2 Installing or exchanging the lens 3 2 4 Installing ...

Страница 28: ...luminator and white light table are exchanged as follows 1 If neither the UV transilluminator or white light table are currently in place inside the instrument proceed to step 4 2 Turn the lever holding the light source to unlock it 3 Pull out the light source carefully along the guide while holding it with both hands 28 ImageQuant LAS 4000 User Manual 28 9607 42 AB 3 Exchanging accessory parts 3 ...

Страница 29: ...hen it should be lowered to tray position 4 using the ImageQuant LAS 4000 Control Software before attaching the lights as described below Hook Latch Epi light base 1 If the tray is at Tray position 4 proceed to step 3 Otherwise close the IDX door 2 Click on Method Tray position set Tray position to 4 and click the OK button 3 Open the IDX door If no Epi light is currently in place inside the instr...

Страница 30: ...i light by pulling it gently outwards 6 Lift up and remove the Epi light 7 Before installing the new Epi light ensure that the latch is sprung open upwards 30 ImageQuant LAS 4000 User Manual 28 9607 42 AB 3 Exchanging accessory parts 3 3 Changing the light sources 3 3 2 Changing the Epi lights ...

Страница 31: ...e it inwards until a click sound is heard 10 Lower the latch to lock the Epi light in place 11 Repeat this process for the opposite side Note The Epi lights are clearly labelled L for the left and R for the right sides Install the Epi lights in matching pairs only If Epi lights with different wavelengths are used the intended image will not be obtained ImageQuant LAS 4000 User Manual 28 9607 42 AB...

Страница 32: ...e ImageQuant LAS 4000 Control Software The following illustration shows the features of the main window of the ImageQuant LAS 4000 Control Software 1 2 3 4 5 6 7 8 9 10 Description No Description No The setting state such as the temperature condition of CCD is displayed 6 Menu bar 1 The state of the Intelligent dark box IDX is displayed 7 Exposure Type Sets the exposure method 2 Method Tray positi...

Страница 33: ...nt with administrator priveleges This is to ensure that necessary folders and files are created 4 2 Preparation for exposure 2 1 Analyzing PC Operation Step Turn on the ImageQuant LAS 4000 computer and peripheral equipment 1 Start up ImageQuant LAS 4000 Control Software A message is displayed until the ImageQuant LAS 4000 is ready 2 Note The power switches of the instrument and PC can be turned on...

Страница 34: ...r trans method 4 3 Calibration Ensure that the ImageQuant LAS 4000 is properly calibrated See Section 5 12 1 CCD Calibration on page 104 and Section 5 12 2 Flat Frame Calibration on page 105 4 4 Placing the sample This section describes how to choose an appropriate sample tray and how to place the sample for exposure 1 Select a sample tray suitable for the type of exposure to be performed Tray Sam...

Страница 35: ... directly on the tray For UV or White Trans trays a Cut out a gel sheet slightly larger than the sample size b Place the gel sheet on the Trans tray Gel sheet c Place the sample on the gel sheet Gel sheet Sample Note The gel sheet can be used repeatedly After use wash it with mild detergent rinse with water then dry well A gel sheet can be reused about 20 times ImageQuant LAS 4000 User Manual 28 9...

Страница 36: ...TIP On the Epi tray there are round dents marked on the tray for positioning the sample Line up the sample using the appropriate dents For NP tray The NP tray is to be used at tray position 3 Note The readable area will be slightly smaller using the F1 8 wide view lens 36 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 4 Placing the sample ...

Страница 37: ...IDX door 4 5 Exposing chemiluminescent samples This section describes how to take images of chemiluminescent samples No illumination is used for this application Place a sample on the Epi tray Put the tray in the ImageQuant LAS 4000 then close the door 5 4 3 6 2 1 ImageQuant LAS 4000 User Manual 28 9607 42 AB 37 4 Operating the ImageQuant LAS 4000 4 4 Placing the sample ...

Страница 38: ...The Method Tray position dialog opens Select Chemiluminescence for Method Select Tray position according to the sample Click the OK button 38 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples ...

Страница 39: ...ly postioned and focus the image Click the Return button TIP Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type 3 ImageQuant LAS 4000 User Manual 28 9607 42 AB 39 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples ...

Страница 40: ...r enter the exposure time manually The exposure time can be set from 0 01 seconds up to 30 hours Note Automaticexposure time setting may not be possible de pending on the sample type and method 40 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 5 Exposing chemiluminescent samples ...

Страница 41: ...should be in place Leave unchecked if this is not nec essary TIP Select Help Sensitivity Resolution This opens the following diagram that de scribes the relation between sensitivity and resolution Click the Start button 6 Result The exposure is started The orange Busy LED lights on the instrument during exposure ImageQuant LAS 4000 User Manual 28 9607 42 AB 41 4 Operating the ImageQuant LAS 4000 4...

Страница 42: ...V trans tray Place the sample on the gel sheet Put the tray into the ImageQuant LAS 4000 and close the door Note The 605DF40 filter is normally used If this filter is currently not in the filter turret it should be installed Refer to Section 3 1 Changing or installing a filter on page 17 Note Ensure that the UV transilluminator is installed For instructions on changing the trans light source see S...

Страница 43: ...ialog opens Select Fluorescence as the Method and choose EtBr from the pull down menu The 605DF40 filter is automatically selected Select the Tray position Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AB 43 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr ...

Страница 44: ... position and focus Click the Return button TIP Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type 3 44 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr ...

Страница 45: ... to 30 hours Note Automaticexposure time setting may not be possible de pending on the sample type and method Select Sensitivity Resolution 5 TIP Select Help Sensitivity Resolution This opens the following diagram that de scribes the relation between sensitivity and resolution ImageQuant LAS 4000 User Manual 28 9607 42 AB 45 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr...

Страница 46: ...he image 7 Click the Complete button The display returns to the main screen TIP If the Method EtBr UV Trans illumination is used the Start button can be clicked and exposure taken even if the temperature setting state of the CCD is Not Ready 46 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 6 Exposing fluorescent samples EtBr ...

Страница 47: ...ut the tray into the ImageQuant LAS 4000 and close the door Note Make sure that sample is suitably placed on the tray for the desired tray position Ensure that a suitable method exists and that the ImageQuant LAS 4000 is properly calibrated See Section 4 16 Creating a new method and performing flat frame calibration on page 78 5 4 3 6 2 1 ImageQuant LAS 4000 User Manual 28 9607 42 AB 47 4 Operatin...

Страница 48: ...thod choose the appropriate fluorescent marker and the ap propriate light source from the pull down menus The appropriate filter is automatically selected Select the Tray position Click the OK button 48 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 7 Exposing fluorescence Epi illumination ...

Страница 49: ...sition and focus Click the Return button TIP Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type 3 ImageQuant LAS 4000 User Manual 28 9607 42 AB 49 4 Operating the ImageQuant LAS 4000 4 7 Exposing fluorescence Epi illumination ...

Страница 50: ...sible de pending on the sample type and method Select Sensitivity Resolution 5 TIP Select Sensitivity Resolution in the Help menu You can display Help that de scribes the relation between sensitivity and resolution Click the Start button The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure 6 50 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the...

Страница 51: ...i light If the White trans tray is available see Section4 9 Exposing dye stained samples and films Trans illumination on page 55 for a more optimal method Place a sample on the Epi tray Put the tray in the ImageQuant LAS 4000 then close the door Note Prior to exposure click on the Digitiza tion Epi illumination tab in Edit Pref erence and make sure that YES is se lected for Invert Pixel data If th...

Страница 52: ... position dialog opens Select Digitization and Epi illumination for Method Select Tray position according to the sample used Click the OK button 52 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light ...

Страница 53: ...nd focus Click the Return button TIP Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type 3 ImageQuant LAS 4000 User Manual 28 9607 42 AB 53 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light ...

Страница 54: ... pending on the sample type and method Select Sensitivity Resolution 5 TIP Select Sensitivity Resolution in the Help menu You can display Help that de scribes the relation between sensitivity and resolution Click the Start button The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure 6 54 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQu...

Страница 55: ...g dye stained samples and films Trans illumination This section describes how to expose dye stained samples using white trans illumination Place a sample on the White trans tray Put the tray in the IDX and close the door ImageQuant LAS 4000 User Manual 28 9607 42 AB 55 4 Operating the ImageQuant LAS 4000 4 8 Exposing dye stained samples and films White Epi light ...

Страница 56: ...ition dialog opens 1 Select Digitization Trans illumination for Method 2 Select Tray position according to the sample used 3 Click the OK button 56 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination ...

Страница 57: ...the focus Click the Return button TIP Click on the image to magnify it Click on the image again to return to the original size Select Precision for Exposure Type 3 ImageQuant LAS 4000 User Manual 28 9607 42 AB 57 4 Operating the ImageQuant LAS 4000 4 9 Exposing dye stained samples and films Trans illumination ...

Страница 58: ...pend ing on the sample type and method Select Sensitivity Resolution 5 TIP Select Sensitivity Resolution in the Help menu You can display Help that describes the relation between sensitivity and resolution Click the Start button The orange Busy LED lights on the instrument and a progress dialog is displayed during exposure 6 58 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQua...

Страница 59: ...ely The data for each image is added to the previous images to provide accumulated exposure data This section describes how to perform this type of experiment Place a sample on the appropriate tray Place the tray in the ImageQuant LAS 4000 then close the door Note Make sure that the tray is placed at the required position ImageQuant LAS 4000 User Manual 28 9607 42 AB 59 4 Operating the ImageQuant ...

Страница 60: ...y position button 1 Result The Method Tray position dialog is opened Select the Method and Tray Position Click the OK button 60 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment ...

Страница 61: ...age to magnify it Click on the image again to return to the original size Select Increment for Exposure Type 3 Select Interval Time unit and enter a numeric value 4 Note Interval Time can be entered in the range of 10 seconds to 2 hours ImageQuant LAS 4000 User Manual 28 9607 42 AB 61 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment ...

Страница 62: ... Help that de scribes the relation between sensitivity and resolution To specify the maximum number of exposures to be taken check the Set Limit check box and specify the number of images 6 62 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 10 Exposing consecutively Increment ...

Страница 63: ...ctivated in Edit Preference under the Increment tab TIP During exposure the gradation conversion curve can be changed and the grada tion adjusted When the Apply All button is clicked the adjusted gradation will be applied to all images 1 2 3 4 5 Description No Description No The changed gradation is ap plied to all images 4 The range can be changed by dragging the mouse 1 Return to initial setting...

Страница 64: ...ables a series of images to be exposed consecutively Each image is recorded separately and data is not accumulated This section describes how to perform this type of experiment Place a sample on the tray that you selected according to the type of a sample Put the tray in the ImageQuant LAS 4000 then close the door Note Make sure that the tray is placed at the required position 6 5 4 3 1 2 7 64 Ima...

Страница 65: ...tion button 1 Result The Method Tray position dialog is opened Select the Method and Tray Position Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AB 65 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition ...

Страница 66: ...age to magnify it Click on the image again to return to the original size Select Repetition for Exposure Type 3 Select Interval Time unit and enter a numeric value 4 Note Interval Time can be entered in the range of 10 seconds to 2 hours 66 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition ...

Страница 67: ...y Help that de scribes the relation between sensitivity and resolution To specify the maximum number of exposures to be taken check the Set Limit check box and specify the number of images 6 ImageQuant LAS 4000 User Manual 28 9607 42 AB 67 4 Operating the ImageQuant LAS 4000 4 11 Exposing repeatedly Repetition ...

Страница 68: ...he gradation conversion curve can be changed and the grada tion adjusted When the Apply All button is clicked the adjusted gradation will be applied to all images 1 2 3 4 5 Description No Description No The changed gradation is ap plied to all images 4 The range can be changed by dragging the mouse 1 Return to initial setting 5 The gradation conversion curvecanbetoggledbetween Linear or Sigmoid 2 ...

Страница 69: ... post exposure state or the File Print This section describes how to print an image Action Step For a screen print first display the window you wish to output 1 Click the Print button or select Print from the File menu Select the output setting for the printer and the type full scale print paper size print or screen print Click the OK button 2 Full scale print Paper size print Screen print The Pri...

Страница 70: ... scope values at both ends of the gradation type of gradation Control Software version number Image Nov Wed 25 15 25 14 2009 Serial 1234567 FUJINON VRF43LMD Cooling 25C Precision Standard 1sec Chemiluminescence Tray Position 1 Range 512 4607 Gradation 512 4607 Linear ImageQuant LAS 4000 mini 1 1 0 17 4 13 Saving exposed images The exposed image can be saved using the Save button displayed in the p...

Страница 71: ...he range of files to be saved 1 Comment When the file is saved in gel format gel a comment can be entered in the file When the file is saved in TIFF format the comment will not be saved 5 Save Display image Max16 Exposure Type Increment Repetition Program All image files displayed in the index view are saved maximum 16 files 2 Make Increment Image Exposure Type Program only Files of multiple expos...

Страница 72: ...e is the total exposure time for the image 2 Select a file format The gel format is a GE Healthcare format that records details of the exposure Such details are not saved in tif format 3 TIP To automatically launch an external application to view images upon saving refer to Launch Applicaton on page 98 Note Special characters such as or other characters that have a special meaning in Windows shoul...

Страница 73: ...he instrument will not be used for a long period it should be turned off Shut down the computer 3 Switch the ImageQuant LAS 4000 off using the power switch 4 4 15 Exposing large sample using wide view lens A larger sample of up to 250 x 250 mm can be exposed by replacing a high sensitivity lens with a wide view lens This section describes how to expose large samples Action Step Install the wide vi...

Страница 74: ...e tray that you selected according to the light source used 2 Put the tray in IDX and close the door 74 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens ...

Страница 75: ...ris and Tray position 2 If FlatFrame Mode is set to manual in Edit Preference click the Next button 3 Select the appropriate Flat Frame file 4 Click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AB 75 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens ...

Страница 76: ...adjust the focus manually Click the Return button TIP Click on the image to magnify it Click on the image again to return to the original size Select Exposure Type 5 Set the Exposure Time or Interval Time 6 76 ImageQuant LAS 4000 User Manual 28 9607 42 AB 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens ...

Страница 77: ...ween sensitivity and resolution Click the Start button 8 Exposure is started Adjust the gradations of the exposed image then save and print the image 9 Click the Complete button The display returns to the main screen ImageQuant LAS 4000 User Manual 28 9607 42 AB 77 4 Operating the ImageQuant LAS 4000 4 15 Exposing large sample using wide view lens ...

Страница 78: ...this to Manual 1 To create a new method click Method Setting in the Option menu The Method Setting dialog opens 2 Select an existing method to change and click the Edit button or to create a new method click Add 3 The Method Setting edit dialog opens Select the appropriate Light Filter and Iris edit the Name for the method and click OK 4 Once the method is added to the list of methods click OK to ...

Страница 79: ... the the appropriate lights and filter are in place To perform a Flat Frame calibration select FlatFrame Calibration in the Option menu 6 The FlatFrame Calibration dialog opens Note Administrator priveleges are required in order to perform a Flat Frame calibration Select the method to calibrate from the drop down list Choose which tray positions to calibrate then click Start to begin the calibrati...

Страница 80: ... for each time set in Interval Time and accumulates images Increment Exposes for each interval time set in Interval Time and displays images for each section Repetition Exposure is made for the desired time and exposure intervals A file is created automatically for each read image and the file of accumulated images is also saved automatically Program 80 ImageQuant LAS 4000 User Manual 28 9607 42 A...

Страница 81: ... is for setting the exposure time When exposure type is precision Auto The exposure time is automatically calculated by histogram analysis ImageQuant LAS 4000 User Manual 28 9607 42 AB 81 5 Software reference guide 5 1 Exposure type ...

Страница 82: ...s to 1 minute Note An image can be exposed for up to 30 hours in Precision mode However the image quality and quantitative performance are only guaranteed for a maximum of 2 hours 5 3 Add digitization image A chemiluminescence and a white light epi illuminated image are exposed by single click operation when this function is set to ON After exposure both chemiluminescence and white epi illuminated...

Страница 83: ... 4 Program settings Program Settings are used to set exposure time and exposure intervals when Program mode is selected as Exposure Type The input settings are saved as a template file 1 12 11 2 3 4 5 6 7 8 9 10 ImageQuant LAS 4000 User Manual 28 9607 42 AB 83 5 Software reference guide 5 3 Add digitization image ...

Страница 84: ... of 0 01 seconds to 30 hours Enter a comment 10 Save the template 4 Select the number of frames between 1 and 16 to be preset 11 Settings will return to the initial settings 5 Select detection sensitivity 12 Folder in which the template is saved 6 Delete a template Navigate to the folder chosen in step 6 above and delete the template file The default locations are as follows For Windows XP C Docum...

Страница 85: ...cus Fast in the View menu The focusing speed then increases This is effective for the alignment of a sample see Pan Focus Fast on page 104 Note When the NP tray is used adjust so that the bottom of a plate is focused Adjustment using a printed paper facilitates focusing Check the tray positions to be exposed in advance 5 6 Start Exposure starts when you click the Start button ImageQuant LAS 4000 U...

Страница 86: ...re a progress bar and ending time are displayed to indicate the progress status of exposure Click Cancel to discontinue exposure The current image is not saved 86 ImageQuant LAS 4000 User Manual 28 9607 42 AB 5 Software reference guide 5 6 Start ...

Страница 87: ...igmoidal 3 Click this tab to magnify the selected image 8 Returns the gradation to the initial setting 4 TIP Exposure is interrupted when you click the Stop button during Precision exposure In this case the image in course of exposure is not saved When you click the Apply All button during magnified image display the gradation changed in the expanded image display state is applied to all magnified...

Страница 88: ...igmoidal 3 Preset conditions 9 Return to initial setting 4 The area where an image will be displayed 10 The changed gradation is applied to all im ages 5 TIP Images for which exposure is completed are automaticallysaved in a file with name format FileName _ PhotographNo _ ExposureTime An image which is the accumulation of all exposed images is saved in a file with name format FileName _ Sum _ Tota...

Страница 89: ...en 4 The range can be changed by dragging the mouse 1 Save image 5 Gradation can be changed by dragging the mouse 2 Return to main screen 6 The gradation conversion curve can be changed linear or sigmoidal 3 ImageQuant LAS 4000 User Manual 28 9607 42 AB 89 5 Software reference guide 5 6 Start ...

Страница 90: ...r screen 8 The gradation conversion curve can be changed linear or sigmoidal 3 The selected image is marked by a yellow frame Click on an image to select it Double click an image or click the Image tab to open the magnified image view 9 The changed gradation is applied to all im ages 4 Returns the gradation to the initial setting 5 5 7 Sensitivity Resolution This function sets the sensitivity and ...

Страница 91: ... Select Sensitivity Resolution in the Help menu The relation between the sensitivity and resolution is displayed 5 8 Method Tray position The window for setting the method and tray position is displayed when you click the Method Tray position button Select a method and tray position most suitable for the sample and click the OK button ImageQuant LAS 4000 User Manual 28 9607 42 AB 91 5 Software ref...

Страница 92: ...e used instead of the separate red green and blue Epi lights Iris Filter Light Option Method F0 85 Through None Chemiluminescence F0 85 Y515 Di Blue 460nm Epi SYBR Green Fluorescence F0 85 510DF10 Blue 460nm Epi GFP F2 8 605DF40 UV 312nmTrans illumi nation EtBr F0 85 Y515 Di Blue 460nm Epi Cy2 F0 85 575DF20 Green 520nm Epi Cy3 F0 85 R670 Red 630nm Epi Cy5 F0 85 IR785 NIR 710nm Epi Dy781 F2 8 L41 U...

Страница 93: ...ged according to the sample size The tray size that can be read varies depending on the tray position Length mm Width mm Position 70 105 1 96 144 2 120 180 3 140 210 4 250 wide view lens 250 wide view lens 210mm 140mm 180mm 120mm Maximum exposure size when a wide view lens is used 144mm 96mm 105mm 70mm 250mm 250mm TIP The display on the main window changes to reflect the current choice of method a...

Страница 94: ...list To create a Flat Frame file see the description of the Flat Frame Calibration function Section 5 12 2 Flat Frame Calibration on page 105 Note Before clicking the OK button be sure to close the instrument door 5 9 File menu Page Setup Opens the settings for the printer for example the direction of paper Print Outputs an image to a printer The image can be printed as a full scale print or scree...

Страница 95: ...Cut Cuts the selected characters This function can be used during manual input of exposure time and interval Copy Copies the selected characters This function can be used during manual input of exposure time and interval Paste Pastes the copied characters This function can be used during manual input of exposure time and interval ImageQuant LAS 4000 User Manual 28 9607 42 AB 95 5 Software referenc...

Страница 96: ...ings can be viewed by clicking the respective item CCD Cooling Sets the CCD temperature Root Folder Sets the folder in which files are saved 96 ImageQuant LAS 4000 User Manual 28 9607 42 AB 5 Software reference guide 5 10 Edit menu ...

Страница 97: ...mation compared to tif format Original Image TIFF File Linear 16bit Gray tif This is a 16 bit TIFF format Window Image TIFF File 8bit Color tif This is a TIFF format that has an eight bit color gradation The changed gradation can be saved directly ImageQuant LAS 4000 User Manual 28 9607 42 AB 97 5 Software reference guide 5 10 Edit menu ...

Страница 98: ...uto Correction data can be selected in the Flat Frame selection screen Manual Launch Applicaton This option can be used to automatically launch an external application when an image is saved 98 ImageQuant LAS 4000 User Manual 28 9607 42 AB 5 Software reference guide 5 10 Edit menu ...

Страница 99: ... to be launched from the drop down list or locate the application using the Select button Check the Activate save and launch function checkbox Print Adjust Adjusts the print output size ImageQuant LAS 4000 User Manual 28 9607 42 AB 99 5 Software reference guide 5 10 Edit menu ...

Страница 100: ...posure is automatically stopped when the amount of satu rated data is too large YES During incremental imaging exposure is continued until the Stop button is pressed NO 100 ImageQuant LAS 4000 User Manual 28 9607 42 AB 5 Software reference guide 5 10 Edit menu ...

Страница 101: ...gitization EPI Description Option Pixel data inversion is performed YES Pixel data inversion is not performed NO ImageQuant LAS 4000 User Manual 28 9607 42 AB 101 5 Software reference guide 5 10 Edit menu ...

Страница 102: ...e automatically shut off and the system will enter power saving mode Auto Range An image whose gradations are adjusted automatically is displayed when exposure type is Increment or Repetition None 102 ImageQuant LAS 4000 User Manual 28 9607 42 AB 5 Software reference guide 5 10 Edit menu ...

Страница 103: ...he background lighter Positive Gray This sets the display color of an image such that absorbing bands are lighter and the background darker Red This sets the display color of an image such that illuminated regions are displayed in red Green This sets the display color of an image such that illuminated regions are displayed in green Blue This sets the display color of an image such that illuminated...

Страница 104: ...orrection file varies depending on the sensitivity exposure time short or long and CCD cooling temperature 1 When CCD Calibration is selected in the Option menu the following window is shown 2 5 3 1 4 Description No Description No Click here to check the boxes for all resolutions for more than 2 hours 4 Click here to check the boxes for all resolutions for up to 2 hours 1 Click on the buttons in t...

Страница 105: ...ition Note Administrator priveleges are required to perform Flat Frame calibration Flat Frame Calibration 1 Select Flat Frame Calibration in the Option menu The window below is then displayed 2 Select a method from the drop down menu at the top 3 Put the displayed tray and Calibration Plate in the instrument 4 Select the condition to be created 5 Click the Start button Note Make sure that an appro...

Страница 106: ... the Start button 5 12 3 Method Setting Method Setting is used to set the content displayed on the Method Tray position screen To delete a method select the method to be deleted and click Delete Note Only user defined methods can be deleted To edit a method select the method to be edited and click Edit Note Only user defined methods can be edited To add a method click Add 106 ImageQuant LAS 4000 U...

Страница 107: ...User Manual Opens the ImageQuant LAS 4000 User Manual in the default pdf browser Getting Started Opens the Getting Started with ImageQuant LAS 4000 manual in the default pdf browser End User License Agreement Opens the End User License Agreement in the default pdf browser Sensitivity Resolution The figure showing the relation between sensitivity and resolution is displayed Click to close the windo...

Страница 108: ...ImageQuant LAS 4000 Control Software The version information of Image Reader is displayed Click to close the window 108 ImageQuant LAS 4000 User Manual 28 9607 42 AB 5 Software reference guide 5 13 Help menu ...

Страница 109: ... 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windows XP Before you begin Log in using a Windows account with administrator privileges Install the USB Control Driver Windows XP Action Step Disconnect ImageQuant LAS 4000 from the computer 1 Open the control panel and select Printers and Other Hardware 2 Click Add Hardware 3 Click the Next button in the Add hardware w...

Страница 110: ...ck the Next button 8 Click the Have Disk button in the Add hardware wizard 9 Insert the ImageQuant LAS 4000 Control Software CD and click the Browse button 10 Select to install the driver from the ImageQuant LAS 4000 Control Software CD 11 110 ImageQuant LAS 4000 User Manual 28 9607 42 AB 6 Installing ImageQuant LAS 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windo...

Страница 111: ... button in the Add hardware wizard 15 Click the Next button again 16 Click the Continue Anyway button in the Hardware Installation dialog 17 Click the Finish button to complete the driver installation 18 ImageQuant LAS 4000 User Manual 28 9607 42 AB 111 6 Installing ImageQuant LAS 4000 Control Software 6 2 Install ImageQuant LAS 4000 Control Software under Windows XP ...

Страница 112: ...ck the Finish button to complete the installation 7 Install ImageQuant LAS 4000 Control Software Windows XP Action Step Insert the ImageQuant LAS 4000 Control Software CD 1 Locate and double click the file ImageQuant LAS 4000 msi 2 In the ImageQuant LAS 4000 InstallShield Wizard click the Next button 3 Read the license text If the license agreement is not acceptable please contact a GE Healthcare ...

Страница 113: ... using a Windows account with administrator privileges Install the USB Control Driver Windows Vista Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Action Step Disconnect ImageQuant LAS 4000 Control Software...

Страница 114: ... and click the Next button 6 Click the Have Disk button 7 Insert the ImageQuant LAS 4000 Control Software CD and click the Browse button 8 Select to install the driver from the ImageQuant LAS 4000 Control Software CD 9 114 ImageQuant LAS 4000 User Manual 28 9607 42 AB 6 Installing ImageQuant LAS 4000 Control Software 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista ...

Страница 115: ...ard Add hardware 13 Click the Next button once again 14 The following warning is displayed Proceed by cliking Install this driver software anyway 15 Click the Finish button in the Add Hardware wizard to complete the installation 16 ImageQuant LAS 4000 User Manual 28 9607 42 AB 115 6 Installing ImageQuant LAS 4000 Control Software 6 3 Install ImageQuant LAS 4000 Control Software under Windows Vista...

Страница 116: ... A successful installation message appears Click the Close button 5 Install ImageQuant LAS 4000 Control Software Windows Vista Note During software installations you may be asked to confirm your actions in a dialog with the text Windows needs your permission to continue Enter an administrator password if prompted then click Continue to proceed with the installation Action Step Insert the ImageQuan...

Страница 117: ... a dialog displays the message An unidentified program wants access to your computer Click Allow 7 Click the Finish button 8 The installation of ImageQuant LAS 4000 Control Software is now completed Start the ImageQuant LAS 4000 Control Software This will ensure that necessary folders and files are created 9 ImageQuant LAS 4000 User Manual 28 9607 42 AB 117 6 Installing ImageQuant LAS 4000 Control...

Страница 118: ... the Remove button 2 Note Correction files created during calibration are required by the ImageQuant LAS 4000 Control Software They are not deleted during the uninstallation and remain in the Data folder of ImageQuant LAS 4000 Control Software folder Uninstalling ImageQuant LAS 4000 Control Software under Windows Vista Action Step Open the control panel and Select Uninstall a program under Program...

Страница 119: ...ays the message An unidentified program wants access to your computer Click Allow 4 Upgrading ImageQuant LAS 4000 Control Software Action Step Uninstall the current version of ImageQuant LAS 4000 Control Software 1 Install the new version of ImageQuant LAS 4000 Control Software 2 ImageQuant LAS 4000 User Manual 28 9607 42 AB 119 7 Uninstalling and upgrading ImageQuant LAS 4000 Control Software ...

Страница 120: ...roducts other than those authorized by GE Healthcare and or problems caused by other equipment Problems and or damage due to moving transport and or falling 8 2 Repairs 1 Before asking for repairs refer to the troubleshooting section in Getting Started with ImageQuant LAS 4000 2 If the problem persists fill in the service report fax sheet at the end of this manual and contact your GE Healthcare re...

Страница 121: ... CCD calibration data from the exposed image Flat Frame In an exposure system using a 2D sensor such as a CCD unevenness due to the lens or lighting system appears on the image By correcting the unevenness an object that radiates light at the same brightness is represented evenly in any section of an exposure area A correction file for correcting unevenness is called a flat frame Under the same co...

Страница 122: ...nce functions Set item Function name Function Precision Exposure Type Exposing for a fixed exposure time Increment Exposure Type Exposing consecutively Repetition Exposure Type Exposing repeatedly 122 ImageQuant LAS 4000 User Manual 28 9607 42 AB A Appendix A 1 Glossary ...

Страница 123: ... Standard High Super Ultra High Binning Super Binning Ultra Binning On Add Digitization Image Exposing a chemilumines cence and a digitized white epi illuminated image by sin gle click operation Chemiluminescence Method Selecting detection method Fluorescence EtBr Fluorescence SYBR Green Fluorescence GFP Fluorescence Cy3 Fluorescence Cy5 Fluorescence Dy781 Fluorescence DAPI Digitization DIA Digiti...

Страница 124: ... tab Changing the folder of file saving Enter size in percent Edit Preference Fine adjusting the print size Print Adjust tab Select and check View Paint saturated data Red Displaying saturated data in red Select and check View Negative Gray Displaying image in Negative Gray Select and check View Positive Gray Displaying image in Positive Gray Select and set conditions for Start Option CCD Calibrat...

Страница 125: ... Chemiluminescence 0 85 Through none Chemiluminescence ECL Plus 0 85 Through none Chemiluminescence Lumi Light Plus 0 85 Through none Chemiluminescence Renaissance 0 85 Through none Chemiluminescence Super Signal 0 85 Through none Chemiluminescence Bright Star 0 85 Through none Chemiluminescence CDP Star 0 85 Through none Chemiluminescence CSPD ImageQuant LAS 4000 User Manual 28 9607 42 AB 125 A A...

Страница 126: ...m Epi Fluorescence SYBRGreen SYPRO Orange 0 85 Y515 Di Blue 460nm Epi Fluorescence SYBRGreen SYPRO tanger ine 0 85 Y515 Di Blue 460nm Epi Fluorescence SYBRGreen FITC 0 85 Y515 Di Blue 460nm Epi Fluorescence SYBRGreen FAM 0 85 510DF10 Blue 460nm Epi Fluorescence GFP EGFP 0 85 510DF10 Blue 460nm Epi Fluorescence GFP ECFP 0 85 575DF20 Green 520nm Epi Fluorescence Cy3 RITC 0 85 575DF20 Green 520nm Epi...

Страница 127: ...tion Trans CBB stain 2 8 Through White Trans Digitization Trans X ray film 2 8 Through White Epi Digitization Epi NBT BCIP 1 Attophos cannot be used for detecting nucleic acid on a nylon membrane Note With regard to patents owned by third parties related to among other things sample preparation we recommend that you consult with a lawyer or patent attorney about obtaining a license from the third ...

Страница 128: ...m or 630 nm RGB Module Epi light 710 nm NIR Epi light Light source Epi and Trans class 1M light source 365 nm UV Epi light 312 nm UV transilluminator 322 x 313 x 87 mm Size 200 x 200 mm UV transmitted filter size White LED White transmitted light table 322 x 313 x 87 mm Size 224 x 161 x 252 mm W H D Camera head 510 x 730 x 480 mm W H D IDX External dimensions 3 4 kg Camera head 49 kg not including...

Страница 129: ...nstrument Specifications of cables required for the use of ImageQuant LAS 4000 100 120 V Voltage 125 V AC 13 A Plug connector SJT3 x 16AWG 60 C Cable Maximum 3 m Power supply cord length 200 240 V Voltage 250 V AC 10 A Plug connector CENELEC OC 3 x 1 0 mm2 70 C Cable Maximum 3 m Power supply cord length ImageQuant LAS 4000 User Manual 28 9607 42 AB 129 A Appendix A 4 Main specifications ...

Страница 130: ...tioning work must be complet ed in advance 3 It is not desirable to have a heat source on the IDX right side face where there is an air intake fan even if the environmental requirements have been met 4 Do not install the equipment near a window to avoid direct sunlight Attach a blind to nearby windows 5 Do not place objects near the power outlet so that you can disconnect the power cable from the ...

Страница 131: ... area where it may get wet or flooded 4 Do not install the equipment in an area where it may be exposed to corrosive gas 5 Do not install the equipment in a dusty area 6 Do not install the equipment in an area constantly or excessively exposed to os cillations or impacts 7 Do not install the equipment in an area exposed to direct sunlight Indoors Operation site 2000 m or lower Maximum operating al...

Страница 132: ...a Business SP1 32 bit 1 GB or more Memory Intel Core 2 Duo processor Processor 80 GB or more HD USB 2 0 USB port version DVD ROM Optical drives 1280 1024 pixels or more Monitor resolution 132 ImageQuant LAS 4000 User Manual 28 9607 42 AB A Appendix A 5 Minimum computer requirements ...

Страница 133: ...X 5 E mail ImageQuant LAS 4000 system information 6 Serial No Camera head Others 7 Analyzing unit type Windows Operating environment Machine model 8 Usage frequency 9 Trouble occurrence frequency 10 Error code and failure description ImageQuant LAS 4000 User Manual 28 9607 42 AB 133 A Appendix A 6 Service report fax sheet ...

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Страница 136: ...o the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany www gelifesciences com quantitative_imaging GE Healthcare UK Limited Amersham Place Little Chalf...

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