GE HEALTHCARE AKTAprime plus, Краткое руководство по эксплуатации

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11-0027-48 AC, 2007-09 • p29
Ordering information
Product Quantity Code No.
HiTrap Blue HP 5 × 1 ml 17-0412-01
HiTrap Desalting 5 × 5 ml 17-1408-01
100 × 5 ml * 11-0003-29
HiPrep 26/10 Desalting 1
(53 ml)
17-5087-01
4
(53 ml)
17-5087-02
Superloop 10 ml 1 18-1113-83
Superloop 50 ml 1 18-1113-84
Superloop 150 ml 1 18-1023-85
* Pack size available by special order
Troubleshooting
High backpressure:
•
Column clogged
– Clean the column according
to instructions. Make sure the sample has been
centrifuged and/or filtered through a 0.45 µm filter.
•
System clogged – Replace the column with a piece
of tubing. Check pressure. If backpressure > 0.3 MPa,
clean system according to manual.
No binding:
•
Check that the correct column is used.
•
Check that the inlet tubing from each buffer is
connected to the correct inlet port.
•
Check that the composition and pH of the buffers are
correct.
•
Check that the sample has been adjusted to binding
buffer conditions.
•
Check that your sample contains target protein.
No elution:
•
Check that the inlet tubing from each buffer is
connected to the correct inlet port.
•
Check that the composition and pH of the buffers are
correct.
•
Use alternative elution conditions according to the
column instructions.
•
Check that your sample contains target protein.
5
Typical result
//
0
1.0
2.0
20 25 15 30 min
AU
280 nm
UV 280 nm
Conductivity
Human albumin
0
50
150
mS/cm
Sample: Human plasma, buffer exchanged to binding buffer
using HiTrap Desalting
Column: HiTrap Blue HP 1 ml
Binding buffer (A1): 20 mM sodium phosphate, pH 7.0
Elution buffer (B): 20 mM sodium phosphate, 2.0 M NaCl, pH 7.0
100
Albumin free
human plasma