FEI Tecnai G2 F20, Руководство по эксплуатационным процедурам

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Using the Falcon II on the Tecnai F20
1. Perform all microscope alignments out of Low Dose
2. Make sure TIA is open
3. Activate Low Dose and load the 50K_recommended.lds file or set up
your low dose as below:
At 50,000x the pixel size is 2.08Å
Search Focus
Exposure (2 sec
integration time)
Magnification
(Detector)
1,700x (Orius) 150,000x (Orius)
50,000x (Falcon)
C2 aperture 100 100 100
Spot size 9 6 6
Defocus -200
µ
m 0-4
µ
m
Dose Rate
(Measured exposure)
on small screen) <0.02 e-/Å
2
/sec
10 e-/Å
2
/sec (2.7 sec)