background image

 

   

   

   

3. STEM mode

 

(a) Find an area of interest at a low magnification (~10 k); 
(b) In the 

STEM 

menu click 

STEM

, Diffraction mode will be activated; press 

the Diffraction button to deactivate the Diffraction mode; 

(c) Under the 

Direct Alignments

, perform Beam Shift, Beam-Tilt Pivot Points X/Y, and 

Rotation Center (turn the outer ring of the objective focus know counter-clock wise to 
stop the wobbling), correct the Condenser astigmatism (triangular shape); 

(e) Press the Diffraction button to return back to the Diffraction mode; 
(f) Insert the appropriate STEM detector. 

  (g) Choose a proper camera length (~ 100 – 150 mm for LM STEM) and spot size; 

(h) Click 

Search 

or 

Preview 

to view the image, and click 

Focus 

and move the red box to 

a contrasty area to adjust focus using the objective focus knob; 

(i) Click 

Acquire 

to obtain an image. In the TIA analysis mode, select the image or panel 

and save them by right-clicking and choosing 

Export Data

 
 
 
 

4. Finishing

 

(a)  Close the column valves (under the 

Setup 

menu, Fig. 1); 

 (b)  Reset the specimen holder position, take it out from the microscope, remove your 

specimen from the holder, and re-insert the holder back; 

(c) Retract the objective and diffraction apertures. 
(d)  Check the web scheduler. If you are the last user for the day, remove the LN

dewar, 

place a paper towel on the dewar stand and immediately go to the 

Vacuum 

panel 

   

under the 

Setup 

menu (Fig. 1), click flap-out menu 

, in the 

Cryo 

tab click 

Cryo

 

Cycle 

(it takes 4 hrs to complete the process); 

Содержание Tecnai G2 F20

Страница 1: ...the ones below and highlighted in green Gun will always be 1 Log 4 Fill the cold trap dewar with LN2 Cover the screen window first Fill the dewar until it is full If you are the first user of the day...

Страница 2: ...ding Remove the objective aperture by sliding the lever to the right Under Setup tab click on Turbo to switch on the turbo pump The turbo pump will start speeding up orange button color and in 3 minut...

Страница 3: ...ol panel 2 Centering the condenser aperture Condenser a Select a C2 aperture 100um for TEM Single particle b At a magnification of 10 000 go to crossover and center the beam using the left trackball c...

Страница 4: ...e phosphor screen using Multifunction knobs X and Y b Beam Tilt Pivot Points X and Y Click on Beam Tilt Pivot Points X and go to crossover Try to minimize the movement of the beam using Multifunction...

Страница 5: ...sert the objective aperture in slide left and using the aperture position knobs on the aperture center it around the center of the un diffracted beam 6 Objective lens stigmation To stigmate the object...

Страница 6: ...k Search or Preview to view the image and click Focus and move the red box to a contrasty area to adjust focus using the objective focus knob i Click Acquire to obtain an image In the TIA analysis mod...

Страница 7: ...the 50K_recommended lds file or set up your low dose as below At 50 000x the pixel size is 2 08 Search Focus Exposure 2 sec integration time Magnification Detector 1 700x Orius 150 000x Orius 50 000x...

Страница 8: ...Appendix Microscope Information Line resolution nm 0 144 Cs objective mm 2 0 Cc objective mm 2 0 Focal length mm 2 7 Detector FEI Falcon II Physical pixel size m 14...

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