background image

 

   

 
4. 

Direct Alignments

: Under the 

Tune 

menu go to 

Direct Alignments 

 

   

 

   

Perform direct alignments at 50,000X and 

close to focus

 on the specimen. 

 
(a) 

Beam Shift alignment

: Click on 

Beam Shift 

and go to crossover. Center the beam in 

the middle of the phosphor screen using Multifunction knobs X and Y. 

   

(b) 

Beam-Tilt Pivot Points (X and Y

): Click on 

Beam-Tilt Pivot Points X 

and go to 

crossover. Try to minimize the movement of the beam using Multifunction knobs X 
and Y. Ideally the beam should look like the picture below with a single bright point 
and minimum movement. If beam moves away from center redo Beam-shift alignment. 
Repeat the same procedure for 

Beam-Tilt Pivot Points Y

 
 
 
 

   

   

   

   

   

   

Bad 

   

   

   

Good 

Содержание Tecnai G2 F20

Страница 1: ...the ones below and highlighted in green Gun will always be 1 Log 4 Fill the cold trap dewar with LN2 Cover the screen window first Fill the dewar until it is full If you are the first user of the day...

Страница 2: ...ding Remove the objective aperture by sliding the lever to the right Under Setup tab click on Turbo to switch on the turbo pump The turbo pump will start speeding up orange button color and in 3 minut...

Страница 3: ...ol panel 2 Centering the condenser aperture Condenser a Select a C2 aperture 100um for TEM Single particle b At a magnification of 10 000 go to crossover and center the beam using the left trackball c...

Страница 4: ...e phosphor screen using Multifunction knobs X and Y b Beam Tilt Pivot Points X and Y Click on Beam Tilt Pivot Points X and go to crossover Try to minimize the movement of the beam using Multifunction...

Страница 5: ...sert the objective aperture in slide left and using the aperture position knobs on the aperture center it around the center of the un diffracted beam 6 Objective lens stigmation To stigmate the object...

Страница 6: ...k Search or Preview to view the image and click Focus and move the red box to a contrasty area to adjust focus using the objective focus knob i Click Acquire to obtain an image In the TIA analysis mod...

Страница 7: ...the 50K_recommended lds file or set up your low dose as below At 50 000x the pixel size is 2 08 Search Focus Exposure 2 sec integration time Magnification Detector 1 700x Orius 150 000x Orius 50 000x...

Страница 8: ...Appendix Microscope Information Line resolution nm 0 144 Cs objective mm 2 0 Cc objective mm 2 0 Focal length mm 2 7 Detector FEI Falcon II Physical pixel size m 14...

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