Lumascope 720-Series Startup Guide
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F. Capturing composite images
1.
Click the Composite icon in left tool bar of Live Image Window. A Composite Color Configuration dialog
box will open.
2.
Select channels (e.g., F1, F2 and F3 for 3-color) and set LED % and Gain % for each based on viewing in
Section II.D above or previous experience. Manual entering of values is recommended because
checking Load Present Values will load only the LED % for each channel while Gain % will be the same
for all channels and correspond to the last determined channel.
3.
Set LED Pre-Snap to 1 second (can be adjusted later). Choose Output Format Type (TIFF
recommended), Filename Root, and Destination Folder (e.g., folder set up on the desktop).
4.
Click OK to start the image capture. Depending on the LEDs selected and Lumaview version, you should
see the LEDs come on in series and/or hear the images being captured (clicking sound).
5.
The composite image made by combining the individual images together will appear in the Main
Window. The image file name at the top of the image includes the full path.
I. Recording live videos
1.
The Video Record icon grabs every frame form the live image and puts them in a folder.
2.
Click red circle icon in left side bar of Live Image Window to open Video Configuration dialog box.
3.
Choose Filename Root, File Format (TIFF recommended), Destination Folder and if time-stamp is
desired. Click OK and images will start to record.
4.
Click red circle icon again to stop. A dialog box will open stating that your images have been saved to
the Destination Folder and how to proceed.
5.
To convert the captured images to video, a variety of options are available including Microsoft
Moviemaker, Apple iMovie, ImageJ, and CellProfiler.
J. Setting up a Protocol
1.
A Protocol allows an automated microscopy method to be configured, saved and run. It consists of
information about the channels, focus, and labware as well as timing and file details.
2.
Clicking the “P” icon opens the Protocol interface.
3.
The first tab of the Protocol is labelled Information and contains Protocol information including
data file path, file root, and image format.
