Lumascope 720-Series Startup Guide
5
Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com
E. Objectives
1.
To install or change objectives, loosen the deck clamp in front until the top deck can be lifted back to
expose the objective. When installing an objective do not overtighten. Remember to retighten the
deck clamp.
F. Calibration
1.
Your Lumascope will need calibrating the first time it is used with any new computer. A 4x objective
and empty 1536-well plate has been included with the LS720 for calibration purposes.
2.
Open the XYZ screen and check the box in the lower left hand corner labelled “Calibrate x/y for
microplate use”. When this is checked, the dialog will expand to show the calibration instructions and
buttons. Shown below is a graphic image of the widened dialog box.
3.
With the 4x objective installed and the 1536 well plate in place, adjust focus until a well is seen.
Moving the focus slider up to about 75% should cause the image to pass through a focus and then this
can be optimized with slider adjustment or the arrow keys. Image brightness can be adjusted as focus
is found.
4.
Now drive the stage with the Relative X/Y Move control until the red cross-hairs are centered on well
A1. This may require you to confirm that you are in the upper left corner well by moving above and left
of A1 to confirm it is indeed the corner well. When you are satisfied that you are centered in A1, press
Accept Calibration.
5.
Uncheck the Calibrate button and choose Select Labware, choose 1536 well plate, and then select
Navigate to well and confirm that the instrument in centered in a few distant wells.
