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Lumascope 720-Series Startup Guide

Etaluma, Inc. | 3129 Tiger Run Court, Suite 112 | Carlsbad, CA 92010 | 760-298-2355 | www.etaluma.com

The next tab is Image

The XYZ tab is where the labware is selected and the wells or positions within that labware are also
selected. Autofocus settings and Tiling menus are also accessed from this tab. These are explained
below.

Содержание Lumascope 720

Страница 1: ...ow to set up the LS720 Section I and start using Lumaview 720 Series Section II It is important that you follow this Guide For assistance please call 760 298 2355 or email support etaluma com Lumascope 720 Startup Guide Lumascope 720 ...

Страница 2: ...c objectives C First step Installing Lumaview 720 Series software on your computer 1 The Lumascope 720 is controlled by the Lumaview 720 Series software program The latest Lumaview version is downloadable from Etaluma s website and must be installed prior to connecting your computer for the first time to the Lumascope Lumaview for LS720 is best run with two monitors one for the settings menus and ...

Страница 3: ...rd USB mini port and the USB A into another USB port on your PC Fig 1 Rear panel connections on LS720beta It is important that the USB cable be connected first between the LS720 and computer before Lumaview is launched This is because Windows needs to load the USB drivers before it can be launched 2 Launch the Lumaview 720 Series software using the desktop icon and allow the Lumascope to be discov...

Страница 4: ...etaluma com b The Lumascope will initialize and connect and a live image will appear In this initialization position the objective is left fully retracted and positioned at the edge of the microplate nest c Click the first icon to adjust image parameters such as channel to be used illumination and Gain and Exposure ...

Страница 5: ... the calibration instructions and buttons Shown below is a graphic image of the widened dialog box 3 With the 4x objective installed and the 1536 well plate in place adjust focus until a well is seen Moving the focus slider up to about 75 should cause the image to pass through a focus and then this can be optimized with slider adjustment or the arrow keys Image brightness can be adjusted as focus ...

Страница 6: ...A Protocol consists of the following tabs Information Acquisition Image XYZ and Image Review Each of these allow one to configure the various settings to construct and save a Protocol for Automated operation A Starting point conditions 1 Lumaview 720 Series has been downloaded to the computer 2 The Lumascope 720 is connected to the computer via the two UBS power communication cables 3 Lumaview 720...

Страница 7: ...ly bright signal Start with Exposure at maximum and Gain as low as possible Increase Illumination to desired brightness For dim samples if Illumination is at maximum increasing the Gain can be used to get enough signal but Gain will increase background as well 3 If desired check false coloring to see images in color If more than one LED is turned on images will be gray scale and signal will be the...

Страница 8: ...ge made by combining the individual images together will appear in the Main Window The image file name at the top of the image includes the full path I Recording live videos 1 The Video Record icon grabs every frame form the live image and puts them in a folder 2 Click red circle icon in left side bar of Live Image Window to open Video Configuration dialog box 3 Choose Filename Root File Format TI...

Страница 9: ...Lumascope 720 Series Startup Guide 9 Etaluma Inc 3129 Tiger Run Court Suite 112 Carlsbad CA 92010 760 298 2355 www etaluma com 4 The Acquisition Tab allows the selection of how images are gathered ...

Страница 10: ...12 Carlsbad CA 92010 760 298 2355 www etaluma com 5 The next tab is Image 6 The XYZ tab is where the labware is selected and the wells or positions within that labware are also selected Autofocus settings and Tiling menus are also accessed from this tab These are explained below ...

Страница 11: ...Lumascope 720 Series Startup Guide 11 Etaluma Inc 3129 Tiger Run Court Suite 112 Carlsbad CA 92010 760 298 2355 www etaluma com 7 Autofocus Settings 8 Tile Selection ...

Страница 12: ... for each well and channel as they are acquired Clicking on a well expands the image 10 The Help Contents files contain descriptions of each screen and field within the menus For questions or comments telephone the phone number below email support etaluma com or submit an inquiry through the Etaluma website at http etaluma com contact An Etaluma representative will be happy to assist you ...

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