Lumascope 720-Series Startup Guide
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6.
Replace the 4x objective with the one you would like to use for your experiment and be sure to select
the new objective under the Configuration drop down.
II. Software: Getting Started with Lumaview 720-Series
The following sections give directions for getting started with Lumaview 720-Series and are intended as a
quickstart guide only. Not all options are given and not all features may be explained in any particular
section. The complete User Guide for Lumaview 720-Series is contained in the Help Section after the
program is opened and is accessible by clicking on F1 or the Help dropdown menu/Contents.
Becoming
familiar with the complete User Guide is a must in order to take advantage of the many features of this
program.
Using Lumaview 720- Summary
Control of your LS720 is divided into Manual operation and Automatic operation.
Manual operation is effected by the top four Icons on the left border. These icons are “Adjust Channels”,
“Position XYZ”, “Snap Picture”, “Snap Composite” and “Record Live Video”. With these controls, one is able
to perform single position, single time point acquisition of single or multiple channel images.
Automatic operation is controlled under the “P” or Protocol icon. Individual tabs in the Protocol screens
allow you to set up automatic versions of the manual configurations determined above. A Protocol consists
of the following tabs: Information, Acquisition, Image, XYZ, and Image Review. Each of these allow one to
configure the various settings to construct and save a Protocol for Automated operation.
A. Starting point conditions
1.
Lumaview 720-Series has been downloaded to the computer.
2.
The Lumascope 720 is connected to the computer via the two UBS power/communication cables.
3.
Lumaview 720-Series is open and has been calibrated with a 4x objective and 1536-well microplate.
4.
Sample to be imaged is present on the Lumascope 720 stage (and fluorescence shroud is over sample if
imaging involves fluorescence).
B. Illuminating the sample for brightfield
CAUTION
: When any LEDs are turned on, do NOT look directly at the light. Be sure to turn off all LEDs at
the end of a viewing session so that any sample remaining on the stage is not photo-bleached.
1.
For viewing in a typically lit area, ambient light is generally adequate to see brightfield. The light should
be as uniform as possible to prevent shadows or other dark or light areas. Overhead fluorescent
lighting can cause uneven brightness including a striped pattern; if observed, move the Lumascope or
partially shade the light to reduce unevenness across the sample. For optimal brightfield, use of the
light source of the Phase Contrast Accessory is recommended (see Section I.B above). Note: Because
the Lumascope 720 CMOS camera is monochromatic, brightfield images will be in black & white.
2.
For viewing in a dark environment such as an incubator, a transmitted light source is needed.
Connecting the Phase Contrast Accessory to the Lumascope allows it to be controlled by Lumaview 720-
Series.
