9
5.
W
hen finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
Hint:
“
Do not release the manifold vacuum pressure by lifting up the corner of 96-well extraction
plate. This will result in deformation of the manifold gasket and will affect the hermetic seal in
future extractions.
”
6.
Load the wash buffer on 96-well extraction plate using multichannel pipette.
7.
Turn the pump ON to apply the vacuum, press slightly on the edges of extraction plate to
engage the proper vacuum seal. Wait till the entire volume passes through the plate and is
collected in Waste Tray.
8.
Repeat the wash steps according to recommendations of kit producers.
9.
When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
10.
Before the elution step remove the 96-well extraction plate and set aside. Disassemble the
manifold and remove the Waste Tray from inside of the manifold (
or throw away the pipette lid
with collected flow through as recommended in Hint section
).
11.
Place the Acrylic spacer block or Adaptor Board on the bottom of the manifold. With the block
or board sitting on the on the bottom, place one 96 Deep Well Collection Plate to collect the
eluate (
follow recommendations of kit producers
).
12.
Assemble the manifold by placing the Upper Chamber on the top of Lower Chamber with 96
Deep Well Collection Plate inside of manifold.
13.
Put back the 96-well extraction plate firmly on the gasket of Upper Chamber so the protruding
column tips sit few millimeters above the 96 Deep Well Collection Plate.
14.
Apply the Elution buffer and elute your samples into the 96 Deep Well Collection Plate. Use
your samples in succeeding PCR analysis OR perform the elution step with centrifugation to
ensure maximal recovery (
follow recommendations of kit producers
).
Hint:
“
You may also remove residual droplets on the bottom of extraction plate by tapping the
top of 96-well extraction plate before releasing the vacuum by Vent.
”
15.
W
hen finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
Vytvořeno: VIJU
Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
Místo uložení: https://www.elisabeth.cz/produkt-zephyrus-vacuum-pump-manifold-system-cz.html
Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění.
Verze: 190620-01
Soubor stažen: 24.06.2020 22:38 online uživatel
Strana 9 z 17
