10
II.
Using the Spin Columns
1.
Put spin column Adaptor Board firmly on the gasket of Upper Chamber.
2.
Replace the number of Luer Stoppers with Luer Connectors on Adapter Board according to
desired number of used spin columns.
Hint:
“
To ensure the proper vacuum level keep the
Luer Stoppers in place on Adaptor Board when
working with fewer spin column
s.”
3.
Insert the spin columns firmly into the Luer connectors. Using same type columns at the same
time will prevent different flow through rate.
4.
Make sure that the vacuum is switched OFF on the vacuum pump.
5.
Load your samples in lysis buffer onto the columns; turn the pump ON to apply the vacuum,
press slightly on the edges of Adaptor Board to engage the proper vacuum seal. Wait till the
entire volume passes through the plate and is collected in Waste Tray.
4.
When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
Hint:
“
Do not release the manifold vacuum pressure by lifting up the corner of the Adaptor Board.
This will result in deformation of the manifold gasket and will affect the hermetic seal in future
extractions.
”
6.
Load the wash buffer onto the columns; turn the pump ON to apply the vacuum, press slightly
on the edges of Adaptor Board to engage the proper vacuum seal. Wait till the entire volume
passes through the plate and is collected in Waste Tray.
7.
Repeat the wash steps according to recommendations of kit producers.
8.
When finished, turn the pump OFF. Slowly and carefully open the Vent on the manifold to
release the residual vacuum to prevent cross-contamination.
9.
Set the Adaptor Board aside and disassemble the manifold, remove the Waste Tray from
inside of the manifold (or throw away the pipette lid with collected flow through as
recommended in Hint section).
10.
Before the elution remove the columns and proceed with centrifugation step to ensure
maximal recovery (follow recommendations of kit producers).
11.
Apply the Elution buffer and elute your samples into collection tubes. Use your samples in
succeeding PCR analysis (follow recommendations of kit producers).
Vytvořeno: VIJU
Instruction for use ZEPHYRUS® Vacuum Pump Manifold System
Místo uložení: https://www.elisabeth.cz/produkt-zephyrus-vacuum-pump-manifold-system-cz.html
Tento dokument je majetkem ELISABETH PHARMACON, spol. s r. o. Je určen ke zveřejnění.
Verze: 190620-01
Soubor stažen: 24.06.2020 22:38 online uživatel
Strana 10 z 17
