5
HTEC-500
5.
System Installation
5-1.
Wiring
Connect the terminal adapter (orange color) to the terminal at the right side of the system. Then
connect the AC cable to the main power supply and turn on the system. “Initializing” appears on the
display, followed by the ECD controller screen. Now you can turn off the system for the next steps
described below.
5-2.
Tubing and Degasser
Pull the tubing’s for the pump rinse, the mobile phase suction and the cell drain out of the HTEC-500
system. Put all the equipment on a level place. Mobile phase and waste bottles should be placed on
the same level as the HTEC-500 (See Fig. 5).
5-3.
Connecting to the Autoinjector and the Autosampler
(Plumbing)
Injectors of the autoinjector (online autoinjector, EAS-20) and/or the autosampler should be set up
downstream to the manual injector. For the autoinjector, connect no. 3 port of the manual injector with
no. 2 port of the autoinjector using 0.125 mm PEEK tubing (red). Then connect no. 3 port of the
autoinjector with the separation column using the same kind of tubing. For the autosampler (model
Alcott 719D or 719AL), connect no. 3 port of the manual injector with no. 2 port of the autoinjector and
no. 3 port of the autoinjector with the separation column.
Please place the HTEC-500 on a horizontal and stable table. The mobile
phase and waste containers should be placed on the same level as the
HTEC-500.
Fig. 5 HTEC-500 Installation
HTEC-500
Mobile Phase
(Always use a glass bottle)
Pump Piston Washing
Syringe for Aspiration
Pump Piston Washing
Ultrapure Water
Mobile Phase Waste
Injector Waste
