13
HTEC-500
8-3.
How to Fix these Errors
A push of the pump key clears the errors. If the same error occurs repeatedly, the problem can be
resolved by referring to the following:
Error 1
The pressure limit value may be set too low. If so, raise the limit value. The optimum pressure limit is
different for each column (see Table 2).
One reason for this error message is that there may be a blockage in the line. Check the precolumn first
by disconnecting the precolumn from the line. If the difference in pressure with the precolumn and
without the precolumn is more than 3 MPa, it is time to exchange the precolumn packing gel and the
upstream filter.
If the error is still not cleared, check the pressure differences of each part as you did for the precolumn
above by going from downstream to upstream of the part. This way you should identify the part which is
blocked. Cut the inlet about a few centimeters if the PEEK tubing is blocked. Use a cutter knife and take
care not to break the inside hole. As the tubing has a constant inner diameter, the blockage factor
cannot go deeper if it comes from upstream. If an autosampler is present, sometimes dregs of vial cup
can cause a blockage at the inlet tubing of the high pressure valve. Please disconnect the tubing at
the first port on the autosampler immediately after the injection port and cut the inlet of the tubing.
Table 2. Column type and recommended pressure limit setting
Column
AC-GEL, E-GEL
SC-3ODS, CA-5ODS,
SC-5ODS (Amino Acids)
PP-ODS
SC-5ODS
Pressure limit setting
8.0
15.0
10.0
12.0
