
Revision: JUNE 2017
62
9. Connecting the HPFM to Roots and Shoots
SAFETY FIRST!
When working with tools, one should always consider safety. Razor blades can cut more than the xylem. So can saw
blades. Being careful cannot protect you all the time. Wear gloves when using sharp objects. Make sure that you are
cutting away from your body (and fingers) as well as away from other people. Wear safety glasses when using a saw
blade. If you are in the field, and you are excising trees (or small parts of large trees), make sure that you wear the
proper equipment, i.e., hard hats, steel-toed boots, when cutting. Other body protection may include sunscreen, long
sleeved shirts full-length pants, and hearing protection. Also, consider the safety of those around you. Be aware of what
you are doing and how it will affect the area around you and the people in that area. Are they wearing the proper safety
attire? If you are in a lab, are they wearing proper protection as well?
Connecting the HPFM to Roots and Shoots
The HPFM, with practice, can be connected to the root, gather data, removed from the root and readied for travel in about
10 minutes. The HPFM can probably be connected to a shoot in less time than a root.
The steps for setting up a root or shoot are:
1. Measure the diameter of the sample where
you plan to excise or cut the sample plant or
tree. Make sure that there are no anomalies
such as grafting scars, limbs, old limb scars,
or excessive damage that might cause
problems with the compression coupling
creating a sealed system. Keep in mind that
the largest object that the HPFM can be
connected to is 50mm with the included
compression couplings. Measure the object
that you wish to sample and make sure that
you are in range before excising the shoot
or trunk! The larger sampled sizes, 40-50mm,
will probably require the bark to be removed.
Any rough surface that will affect the seal of
the compression fitting will need to be
removed or smoothed with sandpaper.
2. Excise the shoot or cut down the trunk. Points to remember before and during the excise procedure are:
a. Rough bark will need to be removed down to the xylem. This can be considered when measuring the sample.
Even if the bark is smooth, water flow could move between the xylem and the bark, or between the bark and
the compression coupling,the bark should be removed.
WARNING!
Do Not Remove the Xylem from the sample.
b. When cutting a root sample, never cut the sample too low to the ground. If you are cutting a shoot sample,
do not cut too close to a major stem. Give yourself plenty of room. Use the rubber compression seal from
the compression coupling you plan to use to check the distance. The point to excise or cut should be:
I. For sizes up to 20mm, leave 2-5mm of root stem above the top of the
rubber compression seal.
Photo 8
Cutting a Stem
Содержание HPFM3
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