4
2. Product Introduction
The DW-K2800 is a measuring (230, 260, 280 nm) nucleic acid analyzer. The
instrument can accurately measure 1~2μL of micro-samples with good repeatability.
Comes with a computer, display and 2 USB ports, built-in software, no need to install,
can connect USB devices such as mouse, keyboard, printer, U disk, wireless network
card. The low concentration detection is more stable, the high concentration detection
range is wider, 200 times that of the conventional UV-Vis spectrophotometer, no
dilution is required, and no baseline calibration is required. The micro-mode sample is
used in a small amount, and the minimum measurement volume is 1 μL, which can be
used to detect nucleic acids, proteins, and the like. All data is automatically saved,
which is convenient for output data statistics, and the software is easy to grasp.
Principle
After the spectrophotometer is in the blank, the instrument automatically records
the spectral result of the blank reference solution and saves it as the light intensity
reference value of the wavelength. When the sample is tested, the light intensity
transmitted through the sample will be recorded. The transmitted light intensity of the
sample and the transmitted light intensity of the blank control are calculated according
to the following formula:
In this way, the absorbance at a specific wavelength can be calculated from the
transmitted light intensity of the sample and the blank.
The relationship between sample concentration and absorbance is determined by
Lambert-Beer's law:
A
=
ε b c
Among: A
＝
Absorbance value
（
A
）
ε
＝
Wavelength dependent molar extinction coefficient
（
unit L/mol*cm
）
b
＝
Optical path
（
unit cm
）
c
＝
Sample concentration
（
unit mol/L
）
Reference solutions, or blank solutions, are usually solvents that melt the
targeting molecule, which has the same pH and ionic strength as the sample solution.
