Dionex IonPac TCC Series, Руководство по эксплуатации продукта

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IonPac TCC-LP1, TCC-ULP1, and TCC-XLP1
Product Manual Page 17 of 17
Doc. No. 034973-06 ©2005 DIONEX September 2005
APPENDIX A
Column Care
A.1 Recommended Operating Pressures
Operating a column above its recommended pressure limit can cause irreversible loss of column performance. The maximum
recommended operating pressure for the IonPac Low Pressure Trace Cation Concentrator Column (TCC-LP1) is 3,000 psi.
A.2 Column Start-up
The column is shipped with 2.5 mM methanesulfonic acid as the storage solution. Flush the column for 30 minutes with
eluent before attempting to concentrate sample. Pump the effluent directly to a waste container while washing the column.
DO NOT pump this effluent through the guard column, analytical column and/or the suppressor.
A.3 Column Storage
The TCC-LP1, TCC-ULP1, TCC-XLP1 columns should be stored in the acid form. Flush approximately 5 mL of any typical
HCl or methanesulfonic acid eluent through the TCC-LP1, TCC-ULP1, and TCC-XLP1.
A.4 Column Cleanup of Polyvalent Cations and Acid-soluble Contaminants
A. Prepare a 500 mL solution of 1 M HCl/0.1 M KCl.
B. Disconnect the guard, analytical columns and the suppressor from the injection valve and the Conductivity
Detector. Disconnect the Gradient Mixer or Cation Trap Column (CTC-1) from the gradient pump. Connect the
TCC-LP1, TCC-ULP1, or TCC-XLP1 Column directly to the gradient pump. Direct the effluent from the TCC-
LP1, TCC-ULP1, or TCC-XLP1 directly to a waste container.
C. Set the flow rate to 1 mL/min.
D. Pump the 1 M HCl/0.1 M KCl solution through the column for 15-30 minutes.
E. Equilibrate the TCC-LP1, TCC-ULP1, or TCC-XLP1 with eluent for 15 minutes at 1 mL/min before resuming
normal operation.
F. Reconnect the cation guard, analytical column and the suppressor between the injection valve and the
Conductivity Detector. Reconnect the Gradient Mixer or Cation Trap Column (CTC-1) between the gradient
pump and the Injection Valve. Resume operation.
A.5 Column Cleanup Of Organic/Cationic Contaminants
A. Prepare a 500 mL solution of 0.1 M HCl/80% acetonitrile.
B. Disconnect the guard, analytical columns and the suppressor from the injection valve and the Conductivity
Detector. Disconnect the Gradient Mixer or Cation Trap Column (CTC-1) from the gradient pump. Connect the
TCC-LP1, TCC-ULP1, or TCC-XLP1 Column directly to the gradient pump. Direct the effluent from the TCC-
LP1, TCC-ULP1, or TCC-XLP1 directly to a waste container.
C. Set the flow rate to 1 mL/min.
D. Pump the 0.1 M HCl/80% acetonitrile solution through the column for 15–30 minutes.
E. Equilibrate the TCC-LP1, TCC-ULP1, or TCC-XLP1 with eluent for 15 minutes at 1 mL/min before resuming
normal operation.
F. Reconnect the cation guard, analytical column and the suppressor between the injection valve and the
Conductivity Detector. Reconnect the Gradient Mixer or Cation Trap Column (CTC-1) between the gradient
pump and the Injection Valve. Resume operation.