54
Release:
09/2008
CyBi
®
-Well
Manual
Operation
elling
through
the
upper
amount
of
freeplay
(3).
If
dispensing
involves
a
greater
path
length
that
the
nominal
volume
would
require,
the
entire
previously
aspirated
volume
will
be
dispensed
from
the
pipetting
tip;
this
is
followed
by
the
amount
of
air
(D2).
A
"Move
pistons
into
zero-position"
command
(5)
will
move
the
pistons
into
their
up-
per
initial
positions
thus
excluding
the
amount
of
lower
freeplay.
This
process
must
be
triggered
with
no
liquid
present,
i.e.
the
lifter
must
be
in
such
a
position
the
tips
cannot
make
contact
with
liquid.
If
liquid
is
aspirated
in
the
process,
this
may
result
in
dosing
errors
or
contamination
of
pistons.
7.5.1
Volume Cycle
Standard cycle:
Aspirate
with
additional
stroke
Dispense
(single
volumes
or
subvolumes)
Residual
dispensing
Move
to
zero-position
Simplified cycle (only dispensing of single volumes):
Aspirate
with
additional
stroke
Dispense
with
additional
stroke
Move
to
zero-position
7.5.2
Waiting Time
Piston
motion
performed
for
aspiration
or
dispensing
will
always
create
some
under-
pressure
or
overpressure,
respectively.
In
order
to
maintain
the
specified
system
preci-
sion,
pressure
equalization
in
the
tips
must
be
allowed
to
finish.
This
is
supported
by
a
specific
"Interruption"
software
command.
A
waiting
time
of
two
seconds
proves
long
enough
with
most
watery
solutions.
The
greater
the
viscosity
of
a
liquid,
the
greater
the
setting
for
delay
must
be.
7.5.3
Precision Performance
The
CyBi
®
-Well
operates
on
the
principle
of
air
displacement,
i.e.
96
or
384
pistons
which
are
mechanically
connected
with
a
common
drive
perform
motion
inside
of
96
or
384
air
spaces
respectively.
Each
of
these
spaces
is
formed
by
a
pipetting
tip
and
an
in-
ternal
seal.
The
sensitivity
of
piston
motion
corresponds
to
one
tenth,
one
hundredth
or
one
thousandth
of
a
micro
liter.
The
achievable
degree
of
precision
is
limited
and
influenced
by:
the
wettability
of
tips,
the
dimensional
stability
of
the
outlet
opening,
the
way
the
system
and
involved
liquids
are
handled.
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