32
Release:
09/2008
CyBi
®
-Well
Initial
Start-Up
&
Routine
Start-Up
Procedure
*Select
a
dye
concentration
so
extinction
is
between
0.4
and
1.2
OD
at
405nm.
Table
9:
Aspiration and test volumes
Tape
the
microplates
after
pipetting
of
the
test
volume
and
before
shaking.
Use
an
orbital
shaker
for
mixing
of
substances.
Table
10:
Mixing times
Measure
extinction
in
vertical
photometer.
Evaluate
measured
data
as
a
preparatory
stage
for
determination
of
CV
value.
Compare
obtained
CV
result
with
CV
values
in
chapter
→
“Technical Data”
.
6.3.2
Accuracy Test
Accuracy
defines
the
degree
to
which
a
measured
dispensed
volume
(mean
value
of
all
readings
measured
for
a
96-well
or
a
384-well
microplate)
is
in
agreement
with
a
pre-
defined
(target)
volume.
Material & preparation:
Laboratory
balance
with
1mg
minimum
resolution.
The
selected
laboratory
balance
must
be
subject
to
regular
calibration
(check
for
calibration
mark).
Lidded
96-well
or
384-well
flat-bottom
microplate.
CyBi
®
-Well 96 /
250
μ
L
CyBi
®
-Well 96 /
25
μ
L
CyBi
®
-Well 384 /
25
μ
L
Aspiration volume
20
μ
L
10
μ
L
10
μ
L
Standard test vol-
ume*
10
μ
L
2
μ
L
2
μ
L
p-nitrophenol dye
solution
125mg/L
in
0.1
N
NaOH
600mg/L
in
0.1
N
NaOH
200mg/L
in
0.1
N
NaOH
96-well microplate
384-well microplate
Orbital shaker of
700rpm
Shake
for
15
min
x
Orbital shaker of
1000rpm
x
Shake
for
10
min
Allow
to
rest
for
30-45
min
Shake
for
10
min
WICHTIG
Since
new
microplates,
typically,
are
vacuum-packed,
a
selected
microplate
must
be
un-
packed
at
least
one
week
before
testing
(the
weight
of
a
new
microplate
may
decrease
or
increase
as
a
result
of
evaporation
and
absorption
respectively).
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