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CyBi

®

-Well

Release:

 

09/2008

III

 

Registered trademarks:

CyBi

®

, CyBio

®

  . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .  CyBio AG, Jena, Gemany

Title

 

to

 

all

 

other

 

trademarks

 

or

 

brands

 

which

 

are

 

referenced

 

in

 

this

 

User

 

Manual

 

belongs

 

to

 

their

 

legal

 

owners.

Copyright

©

Copyright

 

2008

 

CyBio

 

AG.

 

All

 

rights

 

reserved.

 

No

 

part

 

of

 

this

 

documentation

 

may

 

be

 

duplicated,

 

photocopied,

 

saved

 

to

 

a

 

storage

 

system

 

or

 

transferred

 

to

 

electronic

 

media

 

without

 

prior

 

written

 

permission

 

of

 

the

 

publisher.

Publisher

CyBio

 

AG

Goeschwitzer

 

Strasse

 

40

D-07745

 

Jena

Tel:+49

 

-

 

36

 

41

 

/

 

3

 

51-0

Fax:+49

 

-

 

36

 

41

 

/

 

3

 

51-4

 

09

Service

 

Support

Tel:+49

 

-

 

36

 

41

 

/

 

3

 

51-4

 

62

Fax:+49

 

-

 

36

 

41

 

/

 

3

 

51-4

 

75

Ordering number

OL3391-25-001BLE05

Document type

‰

Translation

 

of

 

Original

 

instructions

Serial number

Refer

 

to

 

product

 

nameplate

Artisan Technology Group - Quality Instrumentation ... Guaranteed | (888) 88-SOURCE | www.artisantg.com

Содержание CyBi-Well 384

Страница 1: ...uy your excess underutilized and idle equipment along with credit for buybacks and trade ins Custom engineering so your equipment works exactly as you specify Critical and expedited services Leasing R...

Страница 2: ...CyBi Well y o u r s o l u t i o n s CyBio User Manual Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 3: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 4: ...tocopied saved to a storage system or transferred to electronic media without prior written permission of the publisher Publisher CyBio AG Goeschwitzer Strasse 40 D 07745 Jena Tel 49 36 41 3 51 0 Fax...

Страница 5: ...IV Release 09 2008 CyBi Well 1 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 6: ...es 14 3 8 Requirements On Operating Personnel 14 3 9 Safety Requirements for Transportation Installation 15 3 10 Safety Notes for Operation 15 3 10 1 General 15 3 10 2 Explosion Proofness Fire Prevent...

Страница 7: ...tion Requirements 29 6 1 2 Footprint Requirements 29 6 1 3 Energy Supplies 30 6 2 Configuration Start Up 30 6 3 Function Tests 31 6 3 1 Precision Test 31 6 3 2 Accuracy Test 32 6 3 3 Leak Test 33 7 Ma...

Страница 8: ...61 9 2 4 Tip Magazine 62 9 2 5 Clean Carriage Linear Transporting System 63 9 2 6 Clean Rotary Table 63 9 2 7 Sealing Plate 64 10 Shutting Down 65 11 Accessories Spare Parts 67 11 1 Pipetting tips 67...

Страница 9: ...VIII Release 09 2008 CyBi Well Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 10: ...ystem 19 Fig 5 CyBi Well with circular transporting system 20 Fig 6 Basic CyBi Well Setup Controls 20 Fig 7 CyBi Well rearside view with terminals 21 Fig 8 Pipetting head and CyBi TipTray 23 Fig 9 LCD...

Страница 11: ...X Release 09 2008 CyBi Well Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 12: ...nd a signal word meaning of selected pic tograms and signal words siehe Kapitel Safety Notes They specify the type source and consequences of a hazard giving advice for accident prevention A safety no...

Страница 13: ...conforming use of the CyBi Well pipettor includes that the system is operated by qualified and trained research and laboratory personnel all operating requirements quoted in this User Manual and descr...

Страница 14: ...e limited to repair services or replacement of damaged parts It will ex clude consequential damage of any kind Damage to wear parts and cases of glass breakage will not be covered by this warranty Any...

Страница 15: ...4 Release 09 2008 CyBi Well General Information Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 16: ...of 25 250 L Variation coefficient 1 CyBi Well 96 25 L dosing head Precision for volume range of 5 10 L Variation coefficient 2 Precision for volume range of 10 25 L Variation coefficient 1 CyBi Well...

Страница 17: ...ght details Width x height x depth shallow well without transporting sys tem 220 x 501 5 x 260 mm Width x height x depth deep well without transporting system 220 x 542 5 x 260 mm Width x height x dep...

Страница 18: ...50 60 Hz Inlet fusing 230V T1 6A 115V T3 15A Power consumption 200 VA Airborne sound emission 75dB A Interface RS 232 C Sub D 9 pin Storage operating requirements Storage transportation Allowable ambi...

Страница 19: ...8 Release 09 2008 CyBi Well Technical Data Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 20: ...lly established accident prevention industrial labour protection and environmental protection rules must be considered and duly followed Notices concerning potential hazards do not replace industrial...

Страница 21: ...ling if not avoided CAUTION Indicates a potentially hazardous situation that may result in light or minor injuries or material damage if not avoided NOTE Provides operator advice and other particularl...

Страница 22: ...ote Meaning Comment Warns of dangerous electrical voltage Do not remove system shielding or cover parts Allow repairs to be car ried out only by qualified expert personnel Replace defective fusing wit...

Страница 23: ...te tip mounting device XY stage 2 Dangerous electrical voltage warning note rear side of device 3 Nameplate 4 Fuse 5 Fuse type details 3 6 Mechanical Hazards Fig 2 Hazardous area of pipetting unit 1 T...

Страница 24: ...e fig 2 Lifter see fig 2 carriage ore rotary table see fig 3 Failer to follow these precautions may result in damage to the equipment Improper operation or handling may cause damage to the equipment o...

Страница 25: ...ment or changes in system setup notably such impacting the safety of personnel or the environment 3 8 Requirements On Operating Personnel The CyBi Well system may not be started up operated or maintai...

Страница 26: ...the proper technical condition of the system and its components including any safety devices before they can proceed to action for powering up Notably this applies after a change in an extension to or...

Страница 27: ...s line inlet fuse prevents danger of fire from electrical overload situa tions This fuse must not be short circuited Also make sure that only a fuse of the type specified on the rear panel is used for...

Страница 28: ...components Particular caution should be used when handling leaches acids or organic sol vents because these may reduce the system s lifetime Use only substances that are compatible with the materials...

Страница 29: ...ion of danger make sure that the follow ing requirements are met Operating personnel must be aware of the locations and the proper handling of safety devices accident and hazard alarms as well as firs...

Страница 30: ...channel simultaneously working system requires only four steps to process one 1536X microplate If one or two stackers are added up to fifty microplates can be pro cessed in a fully automated sequence...

Страница 31: ...ane For greater operating convenience the serving unit is rotated by 90 Fig 5 CyBi Well with circular transporting system 4 1 1 System Setup With Operation Control Elements Fig 6 Basic CyBi Well Setup...

Страница 32: ...plate transfer unit 3 Terminal for xy table 4 PSPL voltage supply jack for connection of external components 5 Fusing specifications 6 Fuses 7 Line power connector 115 V 230 V voltage supply convertbi...

Страница 33: ...process 384 well plate formats in a single and 1536 well formats in four steps 4 1 3 CyBi Well Pipetting Unit Motorized motion for pipetting relies on four motors Piston drive Microplate lifter Horiz...

Страница 34: ...he CyBi TipTray should be kept in its desig nated transporting box 4 1 6 Pipetting Piston The CyBi Well operates on the principle of air displacement i e 96 or 384 pistons which are mechanically conne...

Страница 35: ...rs It provides motion of a microplate etc in horizontal direction Zero position of the rotary table is correctly established as part of system initialization 4 1 10 Drop Protector Circular Transportin...

Страница 36: ...g residual volume in pipette tip differential pipetting Discharge residual volume e g into a trough Dispensing Aspirate a total volume as aggregate of predefined sub vol umes Discharge a predefined nu...

Страница 37: ...26 Release 09 2008 CyBi Well Technical Description Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 38: ...During transportation major temperature variations which are likely to cause condensed water mechanical shocks and vibration should be prevented CAUTION Environmental influences mechanical shocks or...

Страница 39: ...quired for a longer period of time it should preferentially be stored in its original packing case Climatic requirements on room facilities for system storage are as follows Temperature range 10 C to...

Страница 40: ...ble horizontal dry and vibration free floor Do not install the system directly beside doors or windows and not in the vicinity of electro magnetic sources of interferences Prevent direct exposure of t...

Страница 41: ...h included packing list when unpacking the various system units Once the system is installed Customer service personnel will test proper function and document the results of testing Refer to Function...

Страница 42: ...is performed at the CyBi Well working in manual mode with an aspira tion volume of 20 L or 10 L Table 9 on page 32 and a test volume of 10 L or 2 L Table 9 on page 32 respectively Dispense the test v...

Страница 43: ...preparation Laboratory balance with 1mg minimum resolution The selected laboratory balance must be subject to regular calibration check for calibration mark Lidded 96 well or 384 well flat bottom mic...

Страница 44: ...stons the pipette tips and the silicon membrane Leak testing is performed by aspiration of a certain volume of dye solution into the pipette tips and watching the liquid level over a time of two hours...

Страница 45: ...ot deviate from the mean value by more than 20 Result of leak testing CyBi Well 96 250 L and CyBi Well 96 25 L There must be no perceivable difference in the filling height of the various pipetting ti...

Страница 46: ...trol software 7 2 Operation Control Elements LCD display Touch screen keypad consisting of Triangle keys Arrow keys Enter key and ESC key Fig 9 LCD display and control elements NOTE For normal CyBi We...

Страница 47: ...ressing this key moves carriage by one place to the left or the right Lifter not in lower limit position Activates X Y positioning of lifter and moves micro plate into one of the following positions A...

Страница 48: ...tion dipsensing or tip change tip mounting program On actuation of the Enter key the carriage slowly moves place 1 into position below the pipetting head in initialization mode In programming mode Abo...

Страница 49: ...ee function groups Manual mode Configuration Statistics A desired function group can be selected via the main menu Fig 10 Menu structure NOTE The system software will retain the latest menu position a...

Страница 50: ...any doubt Consult your service partner in any case of doubt Key Options English or Ger man Selects language via keys and respectively Confirm selection by pressing key Reset drive parameters key Rese...

Страница 51: ...y be effective in manual mode Before calling up this func tion Use triangle keys to move the lifter to a desired height level Confirmation input key Cancel ESC key Click Turns sound for acknowledgemen...

Страница 52: ...approximate discharge rate of 123 l s Note This setting will only be effective in manual mode Range 5 500 rpm Selection arrow keys Confirmation input key Cancel ESC key Table speed Indicates lifter sp...

Страница 53: ...ad Positioning of carriage Precondition lifter in lower limit position Move next place smaller number into position below pipettor Move next place greater number into position below pipettor Lifter ve...

Страница 54: ...put key Preselected volume is aspirated Lifter and carriage control is disabled while aspiration lasts Move reservoir into dispensing position Lower lifter Move carriage Raise lifter Select XY positio...

Страница 55: ...position for aspiration Refer to Positioning com mands at the beginning of this section Aspirate Select Start menu item Selection arrow keys Confirmation input key Preselected volume is aspirated Lif...

Страница 56: ...ing of this section Trigger residual discharge Confirmation input key Differential amount of discharged volume and as pirated volume is discharged The lifter moves down and the system makes a break fo...

Страница 57: ...ed until all subvolumes have been specified Select aspiration volume By de fault the aspiration volume is defined as the sum of subvolumes 2 l This value can be modified if necessary Value arrow keys...

Страница 58: ...bvolume Move reservoir into dispensing position for residual discharge Lower lifter Move carriage Raise lifter Select XY position Refer to Positioning com mands at the beginning of this section Trigge...

Страница 59: ...il all subvolumes have been specified Move reservoir into position for aspiration Lower lifter Move carriage Raise lifter Select XY position Raise lifter move reservoir into position for aspiration Re...

Страница 60: ...t key Pistons move into zero position CAUTION There is danger of crushing or pinching For tip changing a magazine plate must be inserted until mechanical stop position Hold the tip magazine only at it...

Страница 61: ...em will detect a magazine of conforming type and place ment position Fix tip magazine Confirmation input key Number of rinsing cycles Range 1 20 Value arrow keys Confirmation input key Cancel ESC key...

Страница 62: ...nt cycle number and action are output on the display The lifter moves down and the system makes a break for dabbing Dabbing pause Carriage or lifter motion in order to facilitate dab bing Keys and Ter...

Страница 63: ...ion dis pense with additional stroke Aspirate with additional stroke Fig 11 Aspirate with additional stroke This is the recommended standard procedure for precise pipetting Piston motion starts in zer...

Страница 64: ...zero position command 6 triggers upward motion of pistons into their initial positions thus excluding the lower freeplay This process must be triggered with no liquid present i e the lifter must be i...

Страница 65: ...tion 7 5 2 Waiting Time Piston motion performed for aspiration or dispensing will always create some under pressure or overpressure respectively In order to maintain the specified system preci sion pr...

Страница 66: ...This can be prevented by triggering a few initial rinse program cycles until the air inside the tip is saturated with vapor Even after a longer downtime with tips in filled state no liquid will emerg...

Страница 67: ...56 Release 09 2008 CyBi Well Manual Operation Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 68: ...agazine or have defor mation removed 7 System error Inform service partner 8 Failure to find internal zero posi tion of pump system Tip replacement may be able to remove this error Otherwise repair wo...

Страница 69: ...computer program control software 20 Program run was aborted by oper ator 21 Error on calculating motor charac teristic Parameter entries cannot be carried out Select new parameters 22 Error on micro...

Страница 70: ...death Turn system power off and detach power cable from the line socket before you proceed with any kind of maintenance or care Make sure that the system is protected from ac cidental restoration of p...

Страница 71: ...r rotary table x Use mild cleaning agent or disinfectant to clean the tip washing trough x Wash tubes x Replace used tips x Clean tip magazine and perform autoclaving if necessary x Clean all liquid h...

Страница 72: ...n increased probability of failure Check for signs of wear and tear on assem blies under mechanical strain and initiate necessary replacements promptly on identify ing signs of wear and tear Check lif...

Страница 73: ...ep enough Make sure that pistons cannot come into contact with liquid during motion into zero position Repeated faulty operation may lead to excessively high fill levels and a situa tion of the kind a...

Страница 74: ...to mains socket and turn CyBi Well power on at the main switch 3 The system will be re initialized and restored to operating condition 9 2 6 Clean Rotary Table To clean the rotary table proceed as fol...

Страница 75: ...hedding slightly moistened cloth for cleaning 4 Proceed with utmost caution in order to prevent unwanted positional shifts of the sealing plate Otherwise there is danger of plate openings getting clog...

Страница 76: ...and disinfect the system in accordance with instructions for handling of most recently used materials and substances 7 Protect the system from sedimentation of dust 3 The system is shut down now CAUT...

Страница 77: ...66 Release 09 2008 CyBi Well Shutting Down Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 78: ...part of manufacturing Use only accessories and consumables which are recom mended by the Manufacturer No liability will be accepted by the Manufacturer in the event of damage or malfunction due to the...

Страница 79: ...68 Release 09 2008 CyBi Well Accessories Spare Parts Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 80: ...Bi Well will be taken back and disposed by CyBio AG Jena For disposal you are requested to contact the appropriate Customer service operation NOTE Consumable materials must be disposed in compliance w...

Страница 81: ...70 Release 09 2008 CyBi Well Waste Disposal Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 82: ...umes 5 Drive parameters 39 Drop protector 24 E Emergency 18 Energy supply 30 Error list 57 F Fault removal 57 First aid 18 Footprint requirements 29 For connection of external 21 Function 25 Diluting...

Страница 83: ...ion 15 SBS standard 6 Signal words 10 Site requirements 29 Spare parts 67 Special functions 39 Specific system features 52 Standards 9 Standards guidelines 9 Start 37 Starting up 30 Storage 27 Storage...

Страница 84: ...ous radioactive or otherwise hazardous or toxic substances and that no risk or danger is involved in servicing the device Name Description __________________________________ Serial number ____________...

Страница 85: ...74 Release 09 2008 CyBi Well Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 86: ...Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com...

Страница 87: ...1 781 3 76 98 99 Fax 1 781 3 76 98 97 8 James Whatman Court Turkey Mill Ashford Road Maidstone Kent ME 14 5SS UK Phone 44 16 22 66 21 18 Fax 44 16 22 66 41 57 CyBio Japan Co Ltd 4F Meiji Yasuda Seimei...

Страница 88: ...uipment Have surplus equipment taking up shelf space We ll give it a new home Learn more Visit us at artisantg com for more info on price quotes drivers technical specifications manuals and documentat...

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