CORNING Lambda EliteTouch, Инструкция по эксплуатации

Страница: 6 / 17

8 – Corning Lambda EliteTouch Pipettors INSTRUCTION MANUAL – 9
8. Aspiration and Dispensing Instructions
Aspirating Liquid
1. Press the pushbutton to the first positive stop.
Holding the pipettor vertically, immerse the pipet tip
into the sample liquid. The depth to which the pipet
tip is immersed in the sample liquid depends on
the model (for recommended values see Section 7).
If the pipet tip is not immersed to the recommended
depth or if the pipetting pushbutton is rapidly
released, air may enter the disposable pipet tip.
2. Release the pipetting pushbutton slowly and smoothly
to aspirate the sample. Wait one second and then
withdraw the pipet tip from the liquid.
Do not touch the used tip.
Dispensing Liquid
3. Place the end of the pipet tip against the inside wall
of the vessel at an angle of 10° to 40°. Then, press the
pushbutton smoothly to the first stop. Wait one second.
4. Press the pushbutton to the second stop to expel any
remaining liquid. Keep the pushbutton depressed to the
very end, and remove the pipettor by drawing the pipet
tip against the inside surface of the receiving vessel.
5. Release the pushbutton to its starting position.
6. Eject the pipet tip by pressing the tip ejector button.
Remember to change the pipet tip whenever a different
type of liquid is to be sampled.
• The pipettor is equipped with a counter operating mode
switch, the calibration switch located under the CAL sign
on the counter overlay. This switch should only be used
when the pipettor is calibrated (upper position marked
as CAL on the switch).
• Make sure to operate the pipettor slowly and smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration. The recommended immersion depths
are given in the table below:
Model Volume Range [μL] Immersion Depth [mm]
0.1-1 ≤1
1-100 2-3
101-1000 2-4
• The pipettor should be held in a vertical position.
• Change the pipet tip when volume setting is changed
or when a different liquid is to be aspirated.
• Change the pipet tip if a droplet remains on the end
of the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid to
be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
– Press and release the pushbutton slowly and smoothly.
– Never turn the pipettor upside down.
– Never lay the pipettor on its side when there is liquid in
the tip.
• Never force the volume setting beyond its recommended
limits.
• When pipetting liquids with temperatures different from
the ambient temperature, it is recommended to pre-rinse
the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
NOTE: When pipetting acids or corrosive solutions which
emit vapors, it is recommended to disassemble the shaft
and rinse the piston and seal with distilled water after
finishing the pipetting operation.