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Use of the S100x oil-immersion objective

The MSU-400 Series microscopes are equipped with an S100x N.A. 1.25 oil immersion objective. Please follow the 
below instructions on how to use this objective:

1. 

Remove the dust protection cap from the revolving nosepiece to mount the S100x objective

2. 

Focus the image with the S40x objective

3. 

Lock the slide protection handle

4. 

Turn the revolving nosepiece so the S100x objective almost reaches the click-stop

5. 

Put a small drop of immersion oil on the center of the slide

6. 

Now turn the S100x objective so that you feel the click stop

7. 

The front lens is in contact with the immersion oil

8. 

Look through the eyepiece and focus the image with the fine adjustment knobs

9. 

The distance between the lens of the objective and the slide is very small!

10. 

In case there are small bubbles visible, turn the S100x objective a couple of times from left to right so that the 
front of the objective moves in the oil and the bubbles will disappear

11. 

After using the S100x objective, loosen the slide protection handle and turn the table with the course 
adjustment knobs downwards until the front lens doesn’t touch the oil any longer. Clean the front lens of the 
S100x objective

12. 

Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a drop of 
isopropanol.

13. 

Clean the slide after use as well

Illumination

The illumination has the following specifications:

LED

 

   

: 3W NeoLED for binocular and trinocular models.

Power supply 

 

: Primary 100-240 VAC, 50/60 Hz

Fuse specification

 

: 250V 1A

Phase contrast (model 78904-10)

Use of phase contrast

The phase contrast method was designed in 1934 by the Dutchman Frits Zernike to observe very thin or transparent 
objects. This technique uses the fact that light travelling through tissue undergoes a phase shift due to diffraction. By 
recombining the phase shifted light with the background light, a contrasted image appears in the eyepiece.

 

Using the Zernike phase contrast set

Any MSU-400 model with a Zernike phase contrast set comes with the phase contrast condenser and objectives 
already mounted and centered on your microscope. If you suspect misalignment or want to check the alignment 
please see the next point for “centering the phase rings”. The height of the condenser can be adjusted by turning 
the rack and pinion up and down. By doing this the light beam will be focused more on the specimen for maximum 
resolution.

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Содержание 78904-02

Страница 1: ...Installation and Operating Instructions MSU 400 Series Compound Microscopes Models 78904 02 04 07 10 13 78904 02 78904 07 78904 13...

Страница 2: ..._______________________________________________________ 11 Use of the S100x oil immersion objective_________________________________________________________ 12 Illumination____________________________...

Страница 3: ...comes in direct contact with the microscope parts In that case prevention of contamination lies in the handling of the slides as long as the slides are decontaminated before use and are undamaged and...

Страница 4: ...sed disinfection products for laboratories Before returning a microscope for repair or maintenance through a Cole Parmer dealer an RMA return authorization form together with a decontamination stateme...

Страница 5: ...e force when turning the knobs Make sure that the microscope system can dissipate its heat fire hazard Keep the microscope away from walls and obstructions for at least approximately 15 cm Never turn...

Страница 6: ...and S100x objectives are equipped with a spring mount to prevent damage to the front lens and the slide Model Type Objectives Working distance Stand Illumination Power Weight 78904 02 Binocular 4x 10...

Страница 7: ...er adjustment B Trinocular tube K Slide for polarization filter C Microscope head L Nosepiece D Transport handle M Objectives E Height adjustment condenser N Stage with X Y mechanical stage F Slide pr...

Страница 8: ...2 Install the objectives into the objective nosepiece from the lowest magnification to the highest in a clockwise direction from the rear side of the microscope When operating start using the low magn...

Страница 9: ...illumination For optimal contrast and resolution one should follow the below procedure 1 Place a specimen on the object stage and focus using the 4x objective with a fully opened iris diaphragm 2 Tur...

Страница 10: ...e field of view see image below This distance can be set by either pulling the tubes towards each other or pulling them away from each other This distance is different for each observer and thus shoul...

Страница 11: ...aphragm gradually 7 The condenser is centered correctly if the image remains in the center when you open the field diaphragm and inscribed to the field of view The field K hler diaphragm see A in pict...

Страница 12: ...the front lens doesn t touch the oil any longer Clean the front lens of the S100x objective 12 Always clean the front lens of the S100x objective with a piece of lens paper that is moistened with a dr...

Страница 13: ...te the 10 infinity phase contrast objective into the field 10 to match the objective Take the eyepiece out of the tube and insert centering telesc telescope the dark and bright ring images should coin...

Страница 14: ...reen F Fluorescence illuminator G Turret for LEDs Installation steps Take the microscope with fluorescence attachment fluorescence illuminator and head from its packaging Place the head on the fluores...

Страница 15: ...escence filters Blue Green Violet and Ultraviolet mounted in the turret fig 1 D and are indicated as B G V and UV on the turret There is also a brightfield option and it is indicated as 0 on the turre...

Страница 16: ...steps 1 Use objective 10x 2 Push in the field diaphragm switch the one in the front fig 2 A a light spot will be visible in the field of view 3 Adjust the centering if the light spot deviates from th...

Страница 17: ...Cleaning the optics When the eyepiece lens or front lens of the 10x or S40x objective are dirty they can be cleaned by wiping a piece of lens paper over the surface circular movements When this does...

Страница 18: ...roscopes come with a five 5 year factory warranty against manufacturing defects covering labor and parts Warranty does not apply for consumable accessories and parts such as bulbs batteries fuses cord...

Страница 19: ..._ ___________________________________________________________________________________________________ __________________________________________________________________________________________________...

Страница 20: ...2022 Cole Parmer Instrument Company LLC Toll free 1 800 323 4340 Phone 1 847 549 7600 Fax 1 847 247 2929 coleparmer com 1065Y62_M_CP_MSU 400_Series_Compound Microscopes_Manual...

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