Focusing and slide protection
1.
Select the 4x objective and make sure that it is placed correctly in the optical path
2.
Move the stage to the top by using the coarse adjustment knob and focus till the image appears
3.
Rotate the fine focusing knob to sharpen the image
4.
When you perform focusing with a S100x objective, you need to lock the slide protection handle. The slide
protection handle protects the slide by limiting the travel range of the mechanical stage. This way the objectives
will not touch or damage your slides
Adjusting the focusing tension
The tension of the focusing knobs can be adjusted. You can set it
from light to heavy according your own preference. Please note
that when the specimen leaves the focus plane after focusing
or the stage declines out of its own, then you need to adjust the
tension
To tighten the focusing knob (more heavy), rotate the tension
adjustment ring counter-clockwise; to loosen it, please turn it in
the clockwise direction
Eyepieces
Using a binocular (or trinocular) tube is less tiring for the eyes than
a monocular tube. In order to obtain a smooth “compound” image,
we recommend you to go through the below steps:
1. The interpupillary distance
The correct interpupillary distance is reached when one round image is seen in the field of view (see image below).
This distance can be set by either pulling the tubes towards each other or pulling them away from each other. This
distance is different for each observer and thus should be set individually. When more users are working with the
microscope it is recommended to remember your interpupillary distance for a quick set-up during new microscopy
sessions. The MSU-400’s swiveling eyepiece tube can be rotated 360º.
You can select corresponding eye point height according to your own
preference
2. The correct eye point
The eye point is the distance from the eyepiece to the user’s pupil. To
obtain the correct eye point, move the eyes towards the eyepieces until
a sharp image is reached at a full field of view
3. Adjusting the diopter
Using a binocular (or trinocular) tube is less tiring for the eyes than the
use of a monocular tube. In order to obtain the right interpupillary
setting, one should go through the below steps
•
Set diopter adjustment ring to zero
•
Close the left eye and focus the right tube by adjusting the
coarse- and fine adjustment knobs
•
Close the right eye and focus the left tube with the diopter
adjustment ring
This procedure should be followed by each individual user. When
more users are working on the same microscope it is recommended
to remember your own diopter setting for a quick set-up during
new microscopy sessions
Focusing tension
Field of view
before adjustment
slide protection
Field of view
after adjustment
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