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Setting up the FlexiCyte Protocol

Open the ‘Select Protocol’ window opened with the (Fig. 1A)

Select Organism: Mammalian, and Analysis: FlexiCyte, and Media Type:
NC-Slide A2.

Select the FlexiCyte protocol for setting up a new FlexiCyte protocol (Fig.
1B), or select the protocol that should be modified.

Figure 1

Open ‘Select Protocol window (A), and select the FlexiCyte proto-

col (B).

Содержание NucleoCounter NC-3000 FlexiCyte

Страница 1: ...NucleoCounter NC 3000 Quick Guide...

Страница 2: ...erlap 6 Creating and Modifying Graphs 8 Gating and Data Analysis 9 Polygons 9 Quadrants 9 Markers 10 Table plots 12 Identifying the Sub Population in the Image Window 13 Gating Cell Populations for In...

Страница 3: ...Fig 1A Select Organism Mammalian and Analysis FlexiCyte and Media Type NC Slide A2 Select the FlexiCyte protocol for setting up a new FlexiCyte protocol Fig 1B or select the protocol that should be m...

Страница 4: ...eters as described in the help section Editing Image Capture and Analysis Parameters Open Protocol Adaption Wizard Fig 2 located under Tools on the main menu bar Follow the on screen help to select Th...

Страница 5: ...filter combinations The denotation of emission filter as e g Em530 15 means a bandpass filter that allows light of wavelength 530nm 15nm 515nm 545nm to pass Blue LED Green LED Red LED Darkfield Ex365...

Страница 6: ...may be seen on the normal distribution curve Fig 4B If required adjust the exposure time for your sample as appropriate and repeat step 2 6 Step 2 7 should then be repeated for all channels used in t...

Страница 7: ...can be weakly detected in an orange channel To compensate for this fluorescent spillover into neighboring channels a compensation factor can be set for each fluorophore and channel Guidelines for app...

Страница 8: ...peat step 1 7 for each individual fluorophore If multiple samples have been analyzed enter the row number of the sample with the corrected compensation factor into the Master Row box situated in the t...

Страница 9: ...ave been added to the corresponding data row in Plot Manager Double click on the small plot to open it in editing mode Axis scale and parameters can be changed and only those parameters available for...

Страница 10: ...ed Gate menu point and copy to clipboard The plot to which the polygon is to be added should then be opened by double clicking followed by right clicking and selecting Paste Gate Quadrants Open a larg...

Страница 11: ...gth can be adjusted by dragging in the marker line or in the endpoints of the selected red marker Repeat step 2 4 to insert multiple markers into a single histogram Click OK to save markers Markers ca...

Страница 12: ...in editing mode A new text input line will now be present and individual histograms can be colored and named by clicking this line Fig 8 A red cross button will also be present in the large histogram...

Страница 13: ...simply click in the table and write your text To fill in a formula right click a table and fill in your calculation a To select a value from a Quadrant Polygon or Marker use the button To format cell...

Страница 14: ...s so that it appears red Right click and choose the appropriate option for example Add cells inside outside gate to image overlay The events in a scatter plot will be highlighted and the corresponding...

Страница 15: ...ave been set to either Include or Exclude Selecting this option results in display of the sum of all events displayed for the two gates included or excluded Intersection This option is available when...

Страница 16: ...further analysis if desired Save the row with all adjusted compensations markers polygons quadrants and gatings by clicking on the button in the row dialog Right click the file in the file list that...

Страница 17: ...Data can be exported in acs or fcs formats which are compatible with most third party cytometry analysis packages Tip multiple files can be selected in the file browser and can be batch exported by ri...

Страница 18: ...ht click on file to create a PDF report Select the parameters to be visible on the report and the properties of the parameters Optional preview your result Save and or print your report to the default...

Страница 19: ...Figure 12 Figure 11 18 Tip Batch exports can be done from the NucleoView File Browser...

Страница 20: ...n ChemoMetec A S reserves the right to make changes in the product design without reservation and without notification to its users Copyright Notices Copyright ChemoMetec A S 2012 All rights reserved...