9
Gating and Data Analysis
Data analysis will often require that debris or sub-populations of cells are
identified and removed and statistics for the different populations gathered.
Sub-populations of cells can be marked by either quadrants or polygons in scatter
plots and markers in histograms.
•
Polygons
Open a large scatter plot in editing mode by double-clicking on the small
graph.
Select ‘New Polygon’ (Fig. 7).
Place points encircling the population of interest by clicking in the graph
area. Clicking in the grey area outside the scatter plot will remove the last
point added. Click on the first point created to close polygon.
Polygons can also be copied between rows by right-clicking on a selected
red polygon, or use the ‘Selected Gate’ menu point and copy to clipboard.
The plot to which the polygon is to be added should then be opened by
double-clicking followed by right-clicking and selecting ‘Paste Gate’.
•
Quadrants
Open a large scatter plot in editing mode by double-clicking on the small
graph.
Select ‘New Quadrant’ (Fig. 7).
Click at the position in the scatter plot where the centre of the quadrant
should be placed.
While the quadrants are highlighted in red, click the quadrant again. Red
boxes will appear indicating that the quadrant is in editing mode. In this
mode, the centre of the quadrant can be re-positioned and the angle of
the quadrant boundaries can be adjusted.
Quadrants can also be copied between rows by right-clicking on a
selected red Quadrant, or use the ‘Selected Gate’ menu point and copy to
clipboard. The plot to which the quadrant is to be added should then be
opened by double-clicking followed by right-clicking and selecting ‘Paste
Gate’.
