14
Generic Gate
Once compensation factors, quadrants, polygons, markers and gating options
have been defined for a sample, these settings can easily be applied to all
future analyses acquired with a specific protocol.
Check the box associated with the sub-population of interest and the
desired action such as ‘Include’ or ‘Exclude’.
Click ‘Apply’ to show the effect of the gating chosen and ‘OK’ to save. If
gating is not required, check the ‘Disable’ box followed by ‘Okay’.
Note:
if multiple gates have been selected for inclusion or exclusion, they can
be combined by either Union or Intersection of the populations.
•
Union:
this option is available when two gates have been set to either
Include or Exclude.
Selecting this option results in display of the sum of all events
displayed for the two gates (included or excluded).
•
Intersection:
This option is available when two gates have been set to
either Include or Exclude.
Selecting this option results in display of the events displayed for both
of the two gates (included or excluded).
Double-click on the small graph to be analyzed to open the corresponding
larger graph in editing mode.
Click the ‘Gating Setup’ in the upper left corner to open a list of gating
options available for that particular analysis. Gate options are denoted
as ‘P’ for polygon, ‘Q’ for quadrant and ‘M’ for markers followed by a num-
ber indicating the order of creation. The most commonly-used functions
listed are to ‘Include’ or ‘Exclude’ sub-populations but more advanced
settings may also be listed.
