13
Tip:
When viewing a specimen with the 100x objective lens, you can improve the resolving power by placing a small drop
of immersion oil between the specimen and objective lens. For specimen slides that you prepare yourself, always cover the
specimen with a thin piece of glass and place the oil on the cover glass. Do not put the oil directly on the specimen sample.
Adjusting the Focus
The tension of the coarse focus knob can be adjusted to be tighter or looser for your particular needs by moving the tension
ring clockwise or counterclockwise – see Figure 2d.
Figure 2d
Adjusting the Focus Stop
Figure 2e
To change the range of working distance of the stage (4) you need to adjust the limit stop knob. Unlock the limit stop knob
by pushing it counterclockwise. Move the stage upward or downward to the desired position. Then lock the limit-stop
knob by turning it clockwise– see Figure 2e. You will adjust the limit stop knob to prevent an objective lens from hitting
the specimen you are viewing.
Using Filters
To bring out different levels of detail, experiment with changing the color of the back lighting of the specimen. To change
the lighting color, place the blue filter, the green filter, yellow or white filter in the light path by placing one on top of the
illuminator (it will drop in place). You may need to refocus by adjusting the fine focus knob (13) slightly for best viewing.
You should experiment with each of the colors to see the results.
Adjusting the Lighting
Specimens of different size, thickness, and color variations will require different levels of illumination. There
are three ways to change the amount of illumination when viewing a specimen; adjusting the brightness control on the
on/off switch (14) or the illuminator (6), adjusting the Swing condenser (Figure 2b), and adjusting the iris diaphragm (5).
Tension Adjustment
Ring
Limit Stop Knob
Rack &
PinionScrew
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