5.2) Filter cube positioning
When using the MultiSplit to image four colours simultaneously, the example below provides guidance for
dichroic mirror and filter cube positioning according to the internal light path as shown in
Figure 9
.
In this example, four laser lines are used for excitation, however, other light sources (such as LED’s) can be used.
Please contact us (
) if you would like specific filter advice for your requirements.
Laser lines:
405nm
488nm
561nm
640nm
Cube number
Dichroic mirror
Emission filter
(transmitted)
Emission filter
(reflected)
1
T565lpxr-UF2
None
None
2
T635lpxr-UF2
ET 700/75m
ET 600/50m
3
T495lpxr-UF2
ET 525/50m
ET 445/58m
5.3) Polarisation (anisotropy) studies
For polarisation experiments, a Cairn filter cube with an integrated polarising beamsplitter cube* is also available
as an additional accessory. In addition, a rotating 18mm polariser is available to act as a ‘clean-up’ for the
reflected S wave.
The MultiSplit can therefore be configured for a mixture of experiments, such as two-colours in two different
polarisation states.
* Please note the polarising beamsplitter cube is very fragile!
Figure 12: Polarisation components available as additional accessories
5.4) Accessory kits: Neutral Density and Corrector Lenses
Additional accessories are available upon request, which are housed in auxiliary drop-in holders and inserted
into each of the four light paths where required. Blank covers are provided as standard in the absence of these
drop-ins.
