Cairn MultiSplit V2 Скачать руководство пользователя

Страница: 13 / 13

Draw a rectangular region of interest (using ImageJ) around your longest (red) wavelength channel. Click on
‘Select Channel 1’.

Move the same region of interest to the ‘green’ channel and click ‘Select Channel 2’.

Similarly, repeat for the Blue (Channel 3) and Yellow (Channel 4). A four-colour composite overlay will now
be displayed, with individual intensity histograms for each channel.

10.

Each individual image can be nudged using the arrows within the Multi Split window, then saved once
complete.

If you have any questions during installation or use of the MultiSplit, please do not hesitate to contact our Tech

Support Team (

[email protected]

)

Содержание MultiSplit V2

Страница 1: ...ses of this manual all text in bold blue is referenced on subsequent images 1 Mounting to your microscope 1 The MultiSplit is supplied with four detached support feet these should be screwed into the mounts on the base of the unit prior to setup A blue locking ring allows each support leg to be locked off at the correct position 2 Screw your microscope c mount onto the input section of the MultiSp...

Страница 2: ...V controls Auxiliary drop in positions TT Cube 2 magnetic door cover Auxiliary drop in positions TR Cube 3 magnetic door cover Auxiliary drop in positions RR Auxiliary drop in positions RT Figure 2 MultiSplit side panel 1 Figure 3 MultiSplit side panel 2 ...

Страница 3: ...ge 1 Slide the two aperture adjusters one horizontal the other vertical to define your region of interest 2 This region can be locked in place once alignment is complete by turning the aperture handles 3 Once your camera has been mounted following initial alignment the aperture edges should be in sharp focus in your image when partially closed If not please contact us tech cairn research co uk for...

Страница 4: ...airn filter cubes will have this blue handle removed and once inserted into the cube cradles as shown in figure 6 the cradle can be inserted to positions 2 and 3 respectively Please ensure the cube cradles sit flush within the unit and are not angled internal magnets will ensure the cradle locates correctly Figure 6 Fitting cairn cubes 2 and 3 into the cube cradle Figure 7 Cube cradle containing c...

Страница 5: ...al setup a piece of paper held over the camera mounting thread is sufficient to observe the quadrants as each cube is added to the unit Figure 8 Camera mounting thread minus imaging camera 3 1 Light path diagram It is pertinent at this point to consider the internal light path within the MultiSplit and familiarise yourself with the image nomenclature T refers to transmitted and similarly R refers ...

Страница 6: ...d images RT TR and RR Once TT is in the correct quadrant the remaining images can then be positioned in turn Cube 1 Image RT 1 Insert the calibration cube labelled Cube 1 into its correct position note this is the cube with the knurled blue handle this will cause image RT to appear 2 Replace the Cube 1 magnetic door cover 3 Using the 1 5mm Allen key provided re position this image using Cube 1 H V...

Страница 7: ... the quadrant colours are for illustrative purposes only and indicate the image wavelength from high to low Red Yellow Green Blue 4 Obtaining a split image on your camera As the four quadrants are now in the correct position your imaging camera can now be attached to the MultiSplit The silver knurled camera mounting thread on the output tube can be rotated to secure the camera whilst also ensuring...

Страница 8: ...e in our image splitters with minimal image distortion As a result we only recommend the Chroma Ultra Flat UF 2 2mm thick dichroic mirror range The Cairn Filter Cube accepts 26mm x 38mm x 2mm dichroic mirrors and 25mm ring mounted emission filters Other dichroic mirrors will fit however they may not be optimal We are of course happy to provide filter and dichroic mirror advice for your specific ap...

Страница 9: ...F2 ET 525 50m ET 445 58m 5 3 Polarisation anisotropy studies For polarisation experiments a Cairn filter cube with an integrated polarising beamsplitter cube is also available as an additional accessory In addition a rotating 18mm polariser is available to act as a clean up for the reflected S wave The MultiSplit can therefore be configured for a mixture of experiments such as two colours in two d...

Страница 10: ...n used by trial and error for correcting the unwanted effect We recommend starting with the weakest lens longest focal length in the out of focus channel IO137 Focal length 1346mm IO138 Focal length 2019mm IO139 Focal length 4034mm IO140 Focal length 5000mm 1 holder required IO141 Focal length 10 000mm 1 holder required 6 Multi depth Imaging The MultiSplit has two auxiliary drop in positions for e...

Страница 11: ...2 Corrector lens position TT Corrector lens position TR Cube 3 Corrector lens position RT Corrector lens position RR Figure 14 Corrector lens positions for TR and TT Figure 15 Corrector lens positions for RT and RR ...

Страница 12: ...ple C Program Files Micro Manager 2 0 0 gamma1_20190722 mmplugins 3 Obtain a live camera image of your four split channels or alternatively open your saved Tiff file in Micro Manager Please note when overlaying the channels post acquisition the original image must have been saved in Micro Manager the overlay plugin will not work if the file was saved in another software package 4 Click on Plugins ...

Страница 13: ...peat for the Blue Channel 3 and Yellow Channel 4 A four colour composite overlay will now be displayed with individual intensity histograms for each channel 10 Each individual image can be nudged using the arrows within the Multi Split window then saved once complete If you have any questions during installation or use of the MultiSplit please do not hesitate to contact our Tech Support Team tech ...

Результаты поиска

Содержание MultiSplit V2

Отзывы:

Похожие инструкции для MultiSplit V2

Бренды по названию

Популярные бренды

Cairn MultiSplit V2, Руководство пользователя

Результаты поиска

Содержание MultiSplit V2

Отзывы:

Похожие инструкции для MultiSplit V2

Бренды по названию

Популярные бренды