BMG Labtech PHERAstar FS, Руководство по эксплуатации

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BMG LABTECH PHERAstar FS Operating Manual
2009-09-22 0471B0001A 5/26
1 Technical Specifications
Detection Modes
- Absorbance Spectra UV/Vis
- Fluorescence Intensity - including FRET
- Fluorescence Polarization - FarRed™ and Transcreener
®
- Time-Resolved Fluorescence - including DELFIA
®
- TR-FRET - including HTRF
®
, LanthaScreen
®
, LANCE
®
and other TR-FRET assays
- Luminescence (flash & glow) - including BRET
- High-End AlphaScreen
®
Measurement Modes
- Endpoint and kinetic measurements
- Sequential dual excitation measurements
- Simultaneous dual emission measurements
- Ratiometric measurements with real-time ratio
- Top and Bottom reading
- High-Resolution cell layer scanning
Light Source
- High-Energy Xenon flash lamp
- Solid state laser for AlphaScreen
®
- UV Nitrogen 337 nm laser for TR-FRET
Detectors
- Four photomultiplier tubes optimized for reading modes, automatic gain selection,
manual gain available
Optic modules
- Up to six application specific optic modules built-in
Additional optic modules available
Spectral Range
- 230 nm – 750 nm for FI, FP, LUM
- 230 nm – 900 nm for TRF
- 220 nm – 1000 nm for Absorbance
Absorbance
Spectrometer
- Spectral range: 220 to 1000 nm
Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1 nm, 2 nm, 5 nm, 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
Sensitivity
- FI (top): 0.4 pM Fluorescein (10 amol/well) (384)
- FI (top): 2.0 pM Fluorescein (16 amol/well) (1536)
- FI (bottom): 5.0 pM Fluorescein (0.2 fmol/well) (384)
- FP: 1.0 mP SD at 1 nM Fluorescein (384)
- FP: 3.0 mP SD at 1 nM Fluorescein (1536)
- TRF: 25 fM Europium (0.5 amol/well) (384)
- TRF: 100 fM Europium (0.8 amol/well) (1536)
-
HTRF
®
:
Reader Control Kit (Eu) after 18h (384)
Delta F > 1100 % (High Calibrator)
Delta F > 25 % (Low Calibrator)
- LUM: 10 amol/well ATP (384)
- AlphaScreen
®
: < 100 amol * (384)
Microplate Format
- 6 to 1536, user definable
Reading Speed
1 flash: 14 s (384)
1 flash: 27 s (1536)