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BMG LABTECH 

 

PHERAstar 

FS

 Operating Manual 

2009-09-22  

0471B0001A 

15/26 

 

4.5  Description of Optic Modules 

There are single channel optic modules as well as dual channel optic modules for various detection 
technologies. 

Depending on the type of optic module, it can consist of filters, dichroic mirrors, beam splitters and 
polarization film. Emission channel A is always active (whatever measurement principle is in use) and 
Emission channel B is active when dual emission detection is used (for instance as in fluorescence 
polarization, HTRF

®

 and dual luminescence assays).  

The optic module is an important part of the PHERAstar 

FS

 and all parts used in the optic module are 

carefully selected and accurately aligned and tested for optimal performance. 

 

4.5.1  Fluorescence Intensity and TRF Optic Module 

Standard fluorescence intensity optic modules are available in an almost unlimited variety of different 
configurations (contact sales rep with your wishes). All standard fluorescence intensity optics are configured 
as can be seen in figure 18.  
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

Figure 18: Optic module for single  

  channel 

fluorescence 

intensity 

 
The configuration of the filters can be freely chosen from a long list of optimized filters available. 
 

4.5.2   Fluorescence Polarization Optic Module 

The fluorescence polarization optic module consists of many parts, all specially selected and aligned for the 
best performance. Fluorescence polarization optic modules (figure 19) can be customized to fit any 
polarization assay, also at wavelengths other than the standard excitation 485 nm and emission at 520 nm 
and 520 nm. 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 
 

 

Figure 19: Optic module for fluorescence polarization 

Содержание PHERAstar FS

Страница 1: ...Operating Manual Revision A...

Страница 2: ...this manual Suggestions for improving this manual will be gratefully accepted BMG LABTECH reserves the right to change or update this manual at any time The revision number is stated at the bottom of...

Страница 3: ...lications for fluorescence intensity fluorescence polarization time resolved fluorescence HTRF luminescence AlphaScreen and full spectrum absorbance The PHERAstar FS has high end performance in all pl...

Страница 4: ...2 PLATE CARRIER IN OUT BUTTON 12 4 3 PHERASTAR FS SHIPMENT BOX 12 4 4 INSTALLING AND CHANGING OPTIC MODULES 13 4 4 1 BUILT IN BARCODE READER FOR OPTIC MODULES 14 4 5 DESCRIPTION OF OPTIC MODULES 15 4...

Страница 5: ...g modes automatic gain selection manual gain available Optic modules Up to six application specific optic modules built in Additional optic modules available Spectral Range 230 nm 750 nm for FI FP LUM...

Страница 6: ...nal type Wickmann only Dimensions and weight Height 46 cm width 46 cm and depth 51 cm Weight 50 kg max Ambient conditions Operating temperature 15 C to 35 C Storage temperature 10 C to 50 C Humidity o...

Страница 7: ...3 Right side of the PHERAstar FS showing the reagent injection system Press the button on the grip and the optic module door can be slid aside Service lid for laser cartridge Plate carrier in out but...

Страница 8: ...RAstar FS Figure 4 Back of PHERAstar FS Figure 5 Back of PHERAstar FS Main power on off switch Fan Air inlet Miscellaneous labeling such as serial Number CE marking and voltage information Main power...

Страница 9: ...correct operation of the instrument and the safety issues Throughout this manual the word you refers to this trained operator Upon unpacking and positioning the reader make sure to unlock the transpo...

Страница 10: ...ight and must not be moving as it was in the unlocked position Figure 7 Locking the plate carrier Never open the plate carrier door during measuring 3 1 3 Unlocking and Removing the Optic Module Trans...

Страница 11: ...t the power switch on the back of the instrument is in the OFF position Inspect the voltage information on the label next to the power switch see figure 4 to ensure that it corresponds to the local ma...

Страница 12: ...because the amount of air needed to maintain an acceptable inner environment can be acquired more easily through an open plate carrier door 4 2 Plate Carrier In Out Button The plate carrier in out bu...

Страница 13: ...t view and side view When the PHERAstar FS is in its permanent position and powered ON the optic modules can be installed The PHERAstar FS has five positions for optic modules Once the optic module do...

Страница 14: ...r inter changeable type a message will appear informing you manually to type in wavelengths in the optic module table The barcode detection will be triggered by a opening and closing the optic module...

Страница 15: ...tested for optimal performance 4 5 1 Fluorescence Intensity and TRF Optic Module Standard fluorescence intensity optic modules are available in an almost unlimited variety of different configurations...

Страница 16: ...y selected to give the very best performance Figure 20 Optic module for time resolved fluorescence for example HTRF 4 5 4 Luminescence Optic Modules Luminescence assays are mostly towards light of uns...

Страница 17: ...ule to measure light of a specific wavelength Right luminescence optic module to measure light of two specific wavelengths 4 5 5 AlphaScreen Module AlphaScreen is a unique assay technology that measur...

Страница 18: ...priming Refer to chapter 4 6 5 for a detailed description of the priming procedure 4 6 1 Reagent Injectors In order to retain optimal performance it is recommended to follow these guidelines Do not us...

Страница 19: ...are assembled together in a magnetic clamp to minimize dead volume and to guide them to the needle holder The magnetic clamp can easily be removed by gently pulling it away from the instrument In orde...

Страница 20: ...aste container and the needle holder are installed figure 28 The waste container must be placed underneath the needle holder and holds a total volume of max 15 ml Once 15 ml solution volume has been r...

Страница 21: ...g position Hold on to the needle holder and place an external waste container beaker underneath the needle Priming is then initiated either via the Control Software or the respective priming buttons b...

Страница 22: ...RAstar FS Connection to PC whereas the male to female RS232 cable included with the Stacker will connect only the PHERAstar FS to the Stacker If a Stacker is connected with a cable it will automatical...

Страница 23: ...rier in all directions figure 31 It is locked if the plate carrier cannot be moved If the plate carrier can be moved and hence is not locked then either follow the described procedures again or lock i...

Страница 24: ...same fluorescein concentration is an indication of incorrect or inaccurate dimensions of the microplate definition Contact BMG LABTECH service personnel for procedures Figure 33 The various reader off...

Страница 25: ...or the instrument may not be accepted by the service centre or by customs authorities Use suitable disinfectants e g Alcohol 70 Authorized personnel wearing disposable gloves and protective glasses an...

Страница 26: ...been disinfected according to the instructions given in the Operating Manual Name ________________________________________________ Firm ________________________________________________ ______________...

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