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BMG LABTECH 

    

FLUOstar / POLARstar / LUMIstar OPTIMA Operating Manual 

2007-11-19 0413B0001I 

17/23 

 

There are 384-well injection optimized optics with the light guides turned in an angle that makes space for 
the injection to take place. 
 

Note:

   Please be careful when positioning the needle. 

 
 

4.4 Filters 

In the FLUOstar Optima, 4 excitation and 4 emission filters are factory installed, in the POLARstar OPTIMA, 
4 excitation and 5 emission filters are factory installed. (Filter selection varies with instrument configuration. If 
your unit is equipped with luminescence, then a lens will be installed. The absorbance option will also add 
filters.) 

The position of the factory-installed filters can be found in the shipment information that follows the 
instrument. 

After installation of the software, the filter must be typed in the filter table (figure 25). In the Control software, 
the filter table can be reached by choosing ‘Setup | Filter’ (see the software manual for additional 
information). 

 

 

 
 

 
 

 
 
 

 

Figure 25: Filter table with 
example of entered values 

 
 
In the control software you have to type in the filters that are installed (‘Setup | Filters’). It is necessary to 
label the empty position in the emission wheel with a name e.g. “empty”. 

4.4.1  Filter Change and Installation 

All BMG filters have an arrow printed on the side to indicate the direction in which they should be installed. 
The arrow should point in the same direction as the light. If the filter is being installed in the excitation wheel, 
the arrow should point outwards toward the front of the unit.  If the filter is to be placed in the emission wheel, 
the arrow should face inward toward the back of the unit. 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

        

Figure 26: Direction of the light

 

 

Emission 

 

PMT 

Excitation 

 

Flash lamp 

Содержание FLUOstar OPTIMA

Страница 1: ...Operating Manual Revision I...

Страница 2: ...3930 94500 Champigny s Marne Omiya ku 330 0844 Phone 61 3 5973 4744 Phone 33 1 48 86 20 20 Saitama City Japan Fax 61 3 5973 4711 Fax 33 1 48 86 47 07 Phone 81 48 647 7217 australia bmglabtech com fra...

Страница 3: ...ure in the above mentioned modes and additionally in fluorescence polarization mode and simultaneous dual emission The LUMIstar OPTIMA is a luminescence microplate reader that can be upgraded to inclu...

Страница 4: ...12 4 2 2 FLUORESCENCE INTENSITY OPTICS 13 4 2 3 ABSORBANCE OPTICS 13 4 2 4 LUMINESCENCE OPTICS 14 4 2 5 FLUORESCENCE POLARIZATION OPTICS 14 4 2 6 DUAL EMISSION OPTICS 15 4 2 7 HIGH DENSITY OPTICS 15 4...

Страница 5: ...filter positions Gain control Software selectable gain Automatic gain adjustment Plate Carrier Auto lock microplate carrier All microplate formats up to 1536 well in all detection modes Microplates sh...

Страница 6: ...10 OD for 0 2 OD range 0 030 OD for 2 3 OD range Computer interface RS232 38400 baud binary communication protocol Power requirements 100 115 230 V 50 60 Hz Consumption max 205 VA Fuses T2 5A 250V for...

Страница 7: ...ng and positioning the reader make sure to unlock the transport pin section 2 1 Transport Pin before any power connection section 2 2 Power and Communication Connections 2 1 Transport Pin When the ins...

Страница 8: ...ment s power cable and turning the power switch on make sure the transport pin is unlocked section 2 1 Power Connection First check that the power switch on the back of the instrument is in the Off po...

Страница 9: ...A flashing green light means the instrument is busy e g performing a measurement plate in out priming etc A faster flashing 5 flashes per second means an error has occurred Back View On Off Power cabl...

Страница 10: ...8 FLUOstar OPTIMA POLARstar OPTIMA top view of reagent box with combi optic and 2 reagent injectors Top View Reagent Box LUMIstar OPTIMA Transport pin Figure 9 LUMIstar OPTIMA top view of reagent box...

Страница 11: ...ion and Changing of Optics The readers are equipped with quick fix mountings for easy exchange of optics and easy to position spacers for more about spacers see 4 5 No tools are required for changing...

Страница 12: ...ging of Optics For fluorescence measurements excitation enters through the yellow marked light guide and emission is measured through the blue marked light guide For absorbance measurements The grey r...

Страница 13: ...tics Regarding reagent injection be careful when you position the needles in the measurement head see figure 15 to avoid damage to the reagent needles as well as to the optic The optional reagent inje...

Страница 14: ...Luminescence optics Regarding reagent injection be careful when you position the needles in the measurement head see figure 18 to avoid damage to the reagent needles as well as to the optic The option...

Страница 15: ...ptics look like the polarization optics but are not capable of polarization The optic has to be installed in the same way as the polarization optic figure 20 4 2 7 High Density Optics The high density...

Страница 16: ...Well Injection Head A special injection head is required in order to inject into 384 well plates The injection head is installed similarly to an optic see section 4 2 Installation and Changing of Opti...

Страница 17: ...installation of the software the filter must be typed in the filter table figure 25 In the Control software the filter table can be reached by choosing Setup Filter see the software manual for additio...

Страница 18: ...eful not to touch any filters and turn the nut counter clockwise The filter wheel can be pulled straight out of the housing The filter positions are numbered 1 to 8 When installing new filters note th...

Страница 19: ...Fluorescence Polarization Filters The excitation filters can go in any available position in the excitation wheel The emission wheel has two filters of the same wavelength for each channel They shoul...

Страница 20: ...ier see figure 31 spacers needs to be installed under the measurement head see figure 32 There should be enough spacers so that the height of the left side of the plate carrier is slightly higher than...

Страница 21: ...nge barrel Take off the syringe barrel and rinse it with distilled water It may be useful to use the wire syringe cleaners can be found in the service box to scrape particles off the walls In order to...

Страница 22: ...disinfecting the instrument use the following solutions Alcohol 70 Authorized personnel wearing disposable gloves and protective clothing should only perform the disinfection procedure The location sh...

Страница 23: ...disinfected according to the instructions of the operating manual of instrument Name ________________________________________________ Company ________________________________________________ ________...

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