BMG Labtech FLUOstar OPTIMA, Руководство по эксплуатации

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BMG LABTECH FLUOstar / POLARstar / LUMIstar OPTIMA Operating Manual
2007-11-19 0413B0001I 15/23
Position the light guides as follows: right light guide into excitation
position, left light guide into the upper emission position (PMT 1) and
the center light guide connects to the lower emission position (PMT 2).
Only the POLARstar OPTIMA can measure fluorescence polarization.
It is possible to upgrade the FLUOstar OPTIMA to a POLARstar
OPTIMA.
Regarding filters for fluorescence polarization, see chapter 4.4.3.
4.2.6 Dual Emission Optics
The dual emission optics are designed for assays in which you excite at one wavelength and measure two
emission wavelengths simultaneously. This is only possible in the POLARstar OPTIMA because this action
takes two PMT's. The dual emission optics look like the polarization optics, but are not capable of
polarization. The optic has to be installed in the same way as the polarization optic (figure 20).
4.2.7 High Density Optics
The high-density optic has one excitation light guide surrounded by 6 emission
light guides. This optic is designed to minimize cross talk in plate formats such
as 384- and 1536-well plates.
Insert the black mounting piece into the measurement head socket as described
in section 4.2
Installation and Changing of Optics .
The single black light guide connects to the yellow-marked position of the
excitation wheel. The six-bundled light guides have to be inserted into the blue-
marked position of the emission wheel.
Compared to 96-well plates, the 1536-well plates have a low profile. To be able
to read this format the "1536 fluorescence option" is needed.
Injection is not possible in 1536 well plate formats.
Figure 21: High density optics
Figure 20: fluorescence polarization
measurement head