17
Step 3-2.
Add separation buffer into “S” well and add diH
2
O into other wells
※
Fill each well of the buffer tray 80% full
Step 4.
Click “Change Buffer”
Step 5. Allocate the buffer tray into the tray holder
Step 6. Alignment Marker (AM) Preparation: Add at least 20
μ
l Alignment Marker
into the 0.2 ml tube (4 tubes) and add 10
μ
l Mineral Oil on the top of Alignment
Marker
※
Spin down and make sure there is no air bubble in the 0.2 ml PCR tubes
Step 7. Allocate Alignment Marker at “AM” row on the holder
※
Make sure tubes with alignment marker are at the assigned position