Bioneer Exicycler 384, Руководство пользователя

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Revision : 0 (2018-07-05
Exicycler
™
384
NOTE: Example of qPCR protocol
SYBR Green I TaqMan probe
9) Click Incubate tab and enter a temperature in the Temperature field and then a time in the Time field.
a. Incubate sets up a temperature and a time for the thermal block.
Time Increment sets up time increment per cycle.
Temperature Increment sets up temperature increment per cycle.
Ramping Rate sets up a ramping rate.
b. Scan measures fluorescence signals emitted from samples.
c. Goto specifies a starting step of a thermal cycling and a number of cycles.
d. Melting sets up starting temperature and ending temperature for melting curve analysis to
distinguish specific and nonspecific amplification products when SYBR Green is used for Real-
Time PCR.
e. Gradient is used to evaluate an optimum annealing temperature condition for amplification.
f. Store keeps the 384-well thermal block at a set temperature until you stop
Exicycler
™ 384. It is
not recommended to use Store step when it is excessively humid.
g. Pause is used to pause
Exicycler
™ 384 when it is necessary to check samples during
experimentation. The door of
Exicycler
™ 384 will open when the Pause step is inserted in the
protocol file. Click Run to resume the experiment.
h. Hottop sets up a temperature for the heating lid.
NOTE:
Scan is used to detect fluorescence signals from samples. The fluorescence signals are measured for
about 25 seconds at set temperature of the previous incubation step. If the Scan step is not included in
the protocol file, a conventional PCR will be carried out without scanning fluorescence signals.