5061-084-REV-1
LIGHTWAVE 3 & 3+ SPECTROPHOTOMETERS USER’S MANUAL
23
USER INTERFACE
Step 5
Proceed to the second parameters screen by
selecting “Next” using the confirm key.
OR
Return to the Applications screen by selecting
“Cancel” using the escape key.
Step 6
Select the curve fit, “Zero Regression”, “2nd Order
Polynomial”, “Regression”, “Interpolation”, or “Cubic
Spline”, using the left and right arrow keys.
PLEASE NOTE
If only 1 standard sample of known concentration
is used to create the standard curve, only a “Zero
Regression” fit can be selected. If 2 standard
samples are being used to create the standard
curve, only “Zero Regression”, “Regression”,
and “Interpolation” fits can be selected.
Step 7
Press the down arrow, select the source of the
calibration, “Standards”, “Manual”, or “New
Standards”, using the left and right arrow keys.
For “Standards” and “New Standards” calibration,
press the down arrow and select the number of
standard sample replicates, “Off”, “2”, or “3”, using
the left and right arrow keys.
Step 8
Proceed to the Standards screen by selecting
“Next” using the confirm key.
OR
Return to the previous parameters screen by
selecting “Back” using the escape key.
Step 9
Enter a value of up to four significant figures, for
the known concentration of each standard to be
used using the alphanumeric keys.
