5061-084-REV-1
LIGHTWAVE 3 & 3+ SPECTROPHOTOMETERS USER’S MANUAL
19
USER INTERFACE
Step 1
Set the wavelength, 190 to 1100 nm, using the left and
right arrow or alphanumeric keys.
Step 2
Press the down arrow, set the delay time before the
first measurement, 0 to 600 seconds, using the left
and right arrow or alphanumeric keys.
Step 3
Press the down arrow, set the duration of the
observation, 1 to 60 minutes, using the left and right
arrow or alphanumeric keys.
Step 4
Press the down arrow, set the interval between
individual measurements, 5 to 60 seconds, using the
left and right arrow or alphanumeric keys.
Step 5
Proceed to the second parameters screen by selecting
“Next” using the confirm key.
OR
Return to the Applications screen by selecting “Cancel”
using the escape key.
Step 6
Select the mode to define the desired final result,
“Delta A”, “Final A”, or “Slope”, using the left and right
arrow keys.
Kinetics
The Kinetics application is accessed from the Applications screen using the ‘4’ key. It can be used to perform
a series of absorbance (A) measurements over a defined timeframe creating a time-course trace.