Biobase BK-AutoHS96, Руководство пользователя

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Note
：
Consumables used in the experiment, such as 96-well deep-well
plates, 100mL reagent tanks, 5mL cryopreservation tubes, 1000µL
tip, 200µL tip, waste liquid tanks, waste tip bags and other
consumables must be provided by BIOBASE,There may be damage
to the instrument in the use of other types of consumables failure of
the experiment, etc. Please strictly observe this regulation.
4.4.2 Experimental reagent
Before the reagents sub package, please handle them according to BIOBASE
Reagent Operation Manual, such as thawing, mixing, centrifugation, enzyme /
internal standard configuration, etc .;
●
Subpackage for one-step reagents
1
）
Transfer the processed nucleic acid release agent into a 5mL
cryopreservation tube and label it accordingly;
2
）
Transfer the configured reaction mixture PCRmix into 5mL cryopreservation
tubes and mark them accordingly.
●
Subpackage for Magnetic bead reagent
1
）
Transfer the configured magnetic bead extraction solutions 1, 3, and 4 to
100mL reagent tanks respectively and mark them accordingly;
2
）
Mix the configured extraction solution 2 with shaking, centrifuge, transfer it
to a 100mL reagent tank and mark it accordingly;
3
）
Transfer the configured reaction solution PCRmix to a 5mL
cryopreservation tube and mark it accordingly;
●
Subpackage for SUPRall reagent
1 ）
Transfer the configured magnetic bead extraction solutions 1, 2, 3, and 4 to
the 100 mL reagent tanks respectively and mark them accordingly;
2
）
Transfer the configured magnetic bead solution and eluent to
5mLcryopreservation tubes respectively and mark them accordingly;
3 ）
Transfer the configured reaction solution PCRmix into 5mL
Cryopreservation tubes respectivelyand mark them accordingly;
Note
：
Please read the Reagent Operation Manual carefully before the
experiment, and perform reagent pretreatment as required, such as
thawing, mixing, and centrifugation.
Due to the loss of reagents in automated instruments, please
configure the reagents according to the amount of reagents required
to prepare the reagents to ensure that the experiment can be
performed smoothly.