BIO RAD 170-6555, Инструкция по эксплуатации

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13
Installation and Startup
1.5 PREPARING FOR GEL IMAGING
This section discusses the preparation of gels prior to imaging. The table below provides some examples of
how gels must be prepared before they are placed in the Imager tray.
Table 1.
Preparing Gels for Imaging
When preparing DNA and protein gels, follow the guidelines described below:
• Inspect the glass plate for scratches and cleanliness.
A glass plate is required with DNA and Protein gels. Hold each plate up to a light source while varying
your viewing angle. If there are scratches, do not use the plate. Clean off any pieces of gel, finger-
prints, liquids, or lint which will appear as artifacts in the image.
• Place the gel onto the glass plate.
Gently slide the gel onto the glass plate. Align the gel so that it is square on the plate. Remove bubbles
from under the gel by running your finger across the top of the gel. Smooth out the wells so that the flu-
orescent plate will lay flat on top of the gel. Cut off any high ridge on the gel such as those found at the
bottom of the gel.
• Inspect the fluorescent plates for scratches and cleanliness.
The fluorescent plate is required with Protein gels stained with visible dyes and DNA gels with silver
stain. The purpose of the fluorescent bright field plate is to provide a visible (500 nm) light source for
imaging. The UV light excites the fluorophore doped bright field plate wherever there isn’t a stained
band, and it emits a negative image of visible light directly into the camera.
If the fluorescent plate still has a protective coating, remove it while minimizing contact with its surface.
Starting at one end of the gel, gently lower the plate, touching only one end of the plate down first.
Continue to slowly lower the plate onto the gel, pushing out air bubbles as the plate is laid down.
GEL IN CASSETTE GEL
GLASS PLATE
FLUORESCENT PLATE
GLASS PLATE
GEL
FLUORESCENT PLATE
GLASS PLATE
GEL
CARBOHYDRATE GELS
(TAGGED WITH ANTS)
FLUORESCENT GELS
VISIBLE STAINED GELS
(USE PROTEIN SETTINGS)
DNA GELS (STAINED WITH
ETHIDIUM BROMIDE)
DNA GELS
( WITH SILVER STAIN)
PROTEIN GELS
(STAINED WITH VISIBLE DYE)