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Initialization File

[Monosaccharides]

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KeyBand=GlcNAc

•

RF0Band=GlcNAc

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RF1Band=GalNAc

•

BandNames=GlcNAc 50.00,Galactose 50.00,Glucose 50.00,Fucose 50.00,Mannose 50.00,GalNAc
50.00

[Oligosaccharides]

•

KeyBand=G4

•

RF0Band=G12

•

RF1Band=G4

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DP=4

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Quantity=50.0

•

QuantBand=G4

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BandNames=G2,G3,G4,G5,G6,G7,G8,G9,G10,G11,G12,G13,G14

[Lane Descriptions]

•

Monosaccharides=

•

Oligosaccharides=

•

StandardsNames=Monosaccharides,Oligosaccharides

A.5 LAST EXPOSURE TIME

This reflects the last user-entered exposure time.

A.6 BRIGHT FILE LOCATIONS

The location of the Bright Field file (SE1000.brt) is given in the .INI file and therefore, should be placed in
the directory specified in that file. The .INI specification for default Bright Field file is used for the bright field.

A.7 DEFAULT DATA LOCATIONS

File defaults, where files are saved to and important files located are:

•

DefaultDataDir=c:\face\data\

•

DefaultBrightFileDir=c:\face\data\

Содержание 170-6555

Страница 1: ...INSTRUCTION MANUAL Catalog Numbers 170 6555 170 6556 170 6557 170 6558 170 6559 GLYCO DOC GEL IMAGING SYSTEM...

Страница 2: ...1 Files Menu 15 2 2 Image Menu 18 2 3 Bands Menu 21 2 4 Options Menu 24 2 5 Window Menu 25 Section 3 0 Theory of Operations 28 Section 4 0 Maintenance And Troubleshooting 29 4 1 Replacing the UV Ligh...

Страница 3: ...4 Imager Power and Filter Indicators 5 5 Glyco Doc Icon 7 6 Test Plate 8 7 Image Display Control and Status Windows 11 8 SE1000 Camera Control Panel 18 9 Imaging System Functional Diagram 28 10 Inside...

Страница 4: ...eate a potential safety hazard for the user Bio Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than for which it is intended or by modification...

Страница 5: ...de gels as well as DNA and Protein gels and gels stained with visible dyes Figure 1 Glyco Doc Imaging System The Glyco Doc Imaging System provides the following features Integrated high resolution CCD...

Страница 6: ...ur computer Section 1 2 2 is for all users This section discusses how to set up all components in the system 1 2 1 Installing the Camera Circuit Board in a Computer Your host computer must be a standa...

Страница 7: ...ic electricity Make sure the computer is turned off and unplugged Discharge static electricity by touching with the back of your hand a grounded metal surface such as the metal frame of the computer 5...

Страница 8: ...Be sure to remove the small metal antistatic plug from the Imager s computer cable connector This plug protects the connector during shipping Remove and save it for storage or shipment Each cable is...

Страница 9: ...nm Ethidium Bromide and 10 o clock for 500 nm ANTS labeled gels Leave the switch at the 10 o clock position When the Imager is turned on the selected filter will be indicated in the Imager s front pa...

Страница 10: ...isplayed type win a setup If the MS Windows Program Manager is displayed select Run from the File menu and then type a setup 3 Select the desired directory name where the Glyco Doc software files are...

Страница 11: ...eries of self test and configuration status mes sages is displayed followed by the C which is the indication that the system is ready Start the Windows 3 1 interface software by typing win and then do...

Страница 12: ...you did not purchase a computer with the Glyco Doc system Before doing any imaging you must first create a Bright Field file Refer to the chapter on Maintenance for discussion of the purpose of the Br...

Страница 13: ...arge the display window To do so move the mouse so that the cursor is over the bor der of the image area then hold down the left mouse button and move the mouse to enlarge the win dow Then select OK 4...

Страница 14: ...layed in the Band Tables a From the Bands pull down menu select either of the following To locate bands in a region create a Marking Rectangle Then select Find Bands In Marked Rectangle When defining...

Страница 15: ...enu The special cursor allows you to draw a band outline around the band 6 Perform analysis of the Standard Lane a From the Bands menu select Standard Lane Analysis and specify the type of gel b Selec...

Страница 16: ...band in the image and while holding down the left mouse button move the cursor to promote demote the band to the next association level Click on the band value in any band table and while holding down...

Страница 17: ...p of the gel Cut off any high ridge on the gel such as those found at the bottom of the gel Inspect the fluorescent plates for scratches and cleanliness The fluorescent plate is required with Protein...

Страница 18: ...in a standard lane should have band markers a Click on the unwanted band s marker The selected marker is displayed in green b Select Delete from the Bands menu or use the Delete key 2 Select a standa...

Страница 19: ...wn below Image file filename RAW In the Save Files window point and click on the Gel Image RAW checkbox Band data file filename BDF In the Save Files window point and click on the Band Data BDF checkb...

Страница 20: ...and reverse palette settings Print Setup This is an MS Windows function that lets you specify the printer and print quality Import Image This function allows you to import images from other applicati...

Страница 21: ...om the Save File as Type field select the file type supported by your software application The following file types are available along with some of the operating systems and software applica tions wh...

Страница 22: ...u should see little or no yellow in bands to be quantified or in bands that are being used as a quantification standard Saturation is a problem only if it affects the imaging of bands which are to be...

Страница 23: ...tensities adjust the SE1000 Camera Control window s Percent Saturation to limit the percentage of detectors and corresponding pixels that are saturated within a region For example if your band s of in...

Страница 24: ...er image on the screen and a smaller image file This is accomplished by the software which averages the values of four pixels and outputs that averaged value for a single displayed pixel However the i...

Страница 25: ...s function automatically locates and identifies all bands in the image and inserts band markers A band must first be marked before its data is displayed in the Band Tables Bands are marked based on si...

Страница 26: ...elete that band s data from the band data tables A Single Lane To remove all data for a selected lane from the Band Tables Note that the Lanes are re numbered All Bands and Lanes Deletes all band and...

Страница 27: ...n different lanes having similar migration distances as measured in Y axis pixel counts are automatically grouped into Row Associations Band associations can be observed in band tables and in the imag...

Страница 28: ...Lane Center Line The band finding function includes the identification of lanes Along with indicating Lanes by a number you can also indicate lanes by a line drawn through their centers Selecting a la...

Страница 29: ...ng up or down of the gel Band Location Total Gray The Band Location values are the corrected values from the Band Location table The Total Gray value for a band is the total intensity for all pixels i...

Страница 30: ...This table shows the current location measured in pixels along an X Y axis of the cursor in the image area and the grayscale intensity of that pixel Pixel Table Shows the grayscale intensity of every...

Страница 31: ...tting the histogram curve s cursors indicated by red dashed vertical lines to the region of greatest activity which is much narrower than the full range of 0 to 65 535 This function will provide a goo...

Страница 32: ...called blooming and that area of the gel is considered saturated These values serve as the raw data for band analysis Because computer monitors typically display a maximum of 256 shades of gray the c...

Страница 33: ...er cord from the back of the Imager 3 Disconnect the Imager Interface Cable 4 Remove the Imager s Top Cover Slide out the gel holder to reveal two Phillips head screws obscured by the holder s front p...

Страница 34: ...Troubleshooting 8 Perform the previous steps in reverse to install the new tube and close up the Imager 9 Create a new Bright Field file Refer to Section 4 4 Figure 10 Inside the Imager EMISSION FILTE...

Страница 35: ...Remove the Reflector Locate and remove the shiny tent like reflector held in place by two thumb screws Be careful not to bend or distort the reflector You may wish to inspect the Excitation Filter and...

Страница 36: ...V light Cleaning a Filter Cleaning and realigning the mirror Changing the Lens You will need the following materials 1 Clean scratch free Bright Field Plate 1 Test Plate Note Handle the Bright Field P...

Страница 37: ...ta The text to the right of the equals sign is determined during installation and should not be changed unless the Bright Field file is moved The text to the left of the equal sign should always indic...

Страница 38: ...he acquire time is positions is at either 10 o clock or 2 approximately 20 seconds o clock The switch should Neither Filter light is on easily stop at these two but the power light is positions Forcin...

Страница 39: ...ap File is a large hidden file which acts as a buffer for data storage It serves the same function as RAM memory We recommend you have a permanent swap file Generally it needs to be set up only once C...

Страница 40: ...ect A 1 WINDOW DISPLAY DEFAULTS The default settings shown below determine whether various windows appear at startup Unless otherwise specified 0 designates off and 1 designates on at startup Image Hi...

Страница 41: ...k 0 0 0 SaturatedPixsColor 255 255 0 IGRBkColor 0 255 255 IGRBorderColor 192 192 192 IGRRangeMarkColor 255 0 0 ZoomBorderColor 0 128 128 DenBkColor 0 255 255 DenBorderColor 0 0 255 MenuHelpBkColor 0 0...

Страница 42: ...ions pulldown item G2 G3 G4 G5 G6 G7 G8 G9 G10 G11 G12 GalNAc Glucose Galactose Fucose Mannose Sialic Acid GlcNAc KeyBand specifies which name s will appear in the Standard Lane Analysis Dialog Boxes...

Страница 43: ...Oligosaccharides StandardsNames Monosaccharides Oligosaccharides A 5 LAST EXPOSURE TIME This reflects the last user entered exposure time A 6 BRIGHT FILE LOCATIONS The location of the Bright Field fil...

Страница 44: ...ncluded File Formats Exports TIFF WMF BMP PCX GIF and PICT file formats Reports Formats Image band data densitometer and integrated reports Minimum Computer Computer IBM compatible 386 25 MHz computer...

Страница 45: ...Hotel Office Tower 1307 Sumitomo Seimei Kachidoki Bldg Kanalstrasse 17 Phone 516 756 2575 138A Haidian Road 5 3 6 Kachidoki 8152 Glattbrugg 1 800 4 BIORAD Beijing 100086 Chuo Ku Tokyo 104 Phone 01 81...

Страница 46: ...ces Catalog Number Description 170 6555 Glyco Doc Imager 100 240 V includes Glyco Doc imager Glyco Doc analytical software and Glyco Doc imager interface card and cable 170 6557 Glyco Doc Imaging Syst...

Страница 47: ......

Страница 48: ...K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W YfW2 M O g K I 6X e V X V X fW Y W Y...

Страница 49: ...9 31884 0 Fx 089 31884 100 New Delhi India Ph 91 11 461 0103 Fx 91 11 461 0765 Milano Italy Ph 02 21609 1 Fx 02 21609 399 Tokyo Japan Ph 03 5811 6270 Fx 03 5811 6272 Veenendaal The Netherlands Ph 0318...

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