Berthold Crocodile miniWorkstation, Руководство по эксплуатации

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LB 925 Crocodile miniWorkstation 7. Cleaning and Maintenance
84024BA2 Rev. 03, 04/2020 Page 49 of 68
Before and after long breaks:

Use at least the following volumes for liquids others than
distilled water:

for every dispenser: 2ml per sequence step

for the washer-dispenser unit: 8-10 cycles of 1000µl
up to 80ml in total (10ml per needle) per sequence
step.
Use one of the following procedures:

Cleaning with 0.5% bleach:
1. Distilled water
2. 0.5% bleach
3. Distilled water (increased volume)
4. Empty and dry the lines by pumping air

Cleaning with acid/base:
1. Distilled water
2. 0.1 N NaOH
3. Distilled water (increased volume)
4. 0.1N HCl
5. Distilled water (increased volume)
6. Empty and dry the lines by pumping air
Never mix acid and base in the dispenser or washer unit or the
waste container directly. Always rinse with water in between!
Cleaning of the washer aspiration path:

Create a washer protocol with an [Aspirate only] function for
six microplate columns as a minimum.

In cases of distilled water after use of the listed reagents,
increase the number of columns to remove reagent residues.
See the description below.

Do not forget to optimize the aspiration depth for your
microplate.

Fill the respective number of columns of a microplate with
300µl of the solutions listed below and perform the protocol.
Before and after daily use:

Distilled water

Distilled water may remain inside the tubing overnight.