Beckman Instruments Biomek 2000, Руководство пользователя

Страница: 43 / 80

Chapter 3 User’s Guide
Document 609909-AA
Biomek 2000 3-13
Aspirate Rate can be set from 1 to 10. Note that the scale is logarithmic, with lower
numbers being slower. A setting of 6 is quite slow.
Prewet causes the system to pipette extra liquid into and out of the pipette tip before
aspirating the specified volume. This can reduce surface tension between the liquid
and the pipette tip. The actual volume used for prewetting is programmed in the Tool
Edit window.
Tip Touch is used to remove liquid from the outside of the tip after the aspiration by
touching the tip to the side of the well. Tip touch is done at a height which is preset for
each individual type of labware.
Mix settings are on a pop up window which appears when you click Mix Values . The
mix feature aspirates and dispenses for a specified number of times (cycles) to mix
solutions. Selecting
Mix allows you to specify a mix routine function in either the
source or destination range. The Mix occurs prior to aspiration. For many liquids, you
should aspirate from the bottom of the well and dispense near to the top at a rate of 10
to optimize mixing.
Ranges and Patterns are used to set the wells that the tool will visit in the source
labware.
• Range is a contiguous set of wells. You specify the sequence of wells with a
direction of “by Row,” “by Column,” “Box by Row,” or “Box by Column.”
• Pattern is a non-contiguous set of wells. Patterns can be stored and recalled when
needed.
End Action describes what the workstation should do when it has finished with the
first piece of labware. End Action is one of the following:
• Stop stops the transfer after the source range has been completed.
• Repeat Same Labware repeats the source range until the destination range has been
filled. Repeat Same Labware can be used at both the source and the destination.
• Repeat Next Labware completes the range at the source then waits for the next
piece of labware on the worksurface at the same location. (This does not mean that
the source range is repeated at the labware next to the original source.) Move the
next piece of labware into position using either a manual move or an automatic
move (with a Set Shelf command) if a Side Loader is present.
Marks are used to tell the system where the last transfer finished in the range so that
the system can resume the range on subsequent transfers and not return to the
beginning of the range. You can use the following options for setting marks:
• Use no Marks is the default setting.
• Set a New Mark marks the well where the next pipetting function at that piece of
labware is to begin.
• Use an Existing Mark starts the system at the current mark. This does not move the
mark after the transfer is complete.
• Use then Set New Mark starts the system at the current mark and moves the mark
after the transfer.