Beckman Instruments Biomek 2000, Руководство пользователя

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User’s Guide Chapter 3
Document 609909-AA
3-12 Biomek 2000
Information pertaining to the source labware (used for aspiration) is on the left of the
screen, and information pertaining to the destination labware (or destination) is on the
right. Transfer volume, tool and tip options are located in the center of the screen. The
fields that you will edit on this screen are summarized below:
Source
Aspirate Height sets the height the tip goes into the labware. The range is from
-10% to 110%, where 0% is at the bottom of the labware well and 100% is at the top.
Some tool and tip combinations cannot go all the way to the bottom of certain types of
labware, in which case the range will be limited by BioWorks.
Liquid Level options are Fixed, Liquid Level Sensing, or Liquid-Level Tracking.
• Fixed means that on each visit to the source labware, the tip will go to the
programmed aspirate height.
• Sensing will determine where the liquid is in a well, then aspirate the volume just
below the surface (miniscus). Sensing is only available with the P200L and
P1000L tools. These tools sense the liquid level and automatically determine the
aspirate height.
Sensing is used on each visit to a well when a new tip is used, and on the first visit
to the well if the same tip is used. Subsequent visits use tracking to calculate the
proper depth. When Sensing is selected, the Tip Depth box becomes active. Use
this to set an aspirate depth below the depth calculated for the aspiration volume.
Enter the number of millimeters below the liquid level at which you wish to use
the Biomek to aspirate in the Depth field. This may be useful to aspirate the lower
level of a two-phase solution.
Sensing also detects whether or not a tip is present, and will also reject “dirty” tips.
Always use clean tips with liquid sensing.
Hint
You should usually use a tip depth of 5mm to 10mm to ensure that
only liquid is aspirated from the well. A setting of 0mm can cause
problems due to aspiration of air.
• Tracking means that on the first visit to the well, the tip goes to the programmed
height. On subsequent visits, the system will calculate the distance to move down
based on the volume of liquid it aspirated on the previous visit, and the shape of
the labware. This feature is useful if a full tube is to be emptied with multiple
aspirations, because no liquid will remain on the tip, and the liquid will not be
spilled out on the first visit.
Hint
Set the first height well below the actual level to ensure that all liquid
is aspirated, and to avoid aspiration of air.